Cloned (Comment) | Organism |
---|---|
in somatic cells, LDH forms homotetramers and heterotetramers that are encoded by two different genes: LDHA (skeletal muscle type, M) and LDHB (heart type, H) | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxychloroquine | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten and Hanes-Woolf kinetic analysis and thermodynamics of isozymes H4, M4, and H2M2, overview. The Km values for heteroterameric H2M2-mediated catalysis of pyruvate or lactate are between those for the homotetrameric isozymes, M4 and H4, whereas the Vmax values are similar. The Km and Vmax values for H2M2-mediated catalysis of NADH are not significantly different among LDH isozymes. The values for activation energy and van't Hoff enthalpy changes for pyruvate reduction of H2M2 are between those for the homotetrameric isozymes. The temperature for half residual activity of H2M2 is closer to that for M4 than for H4 | Sus scrofa | |
0.0026 | - |
(S)-lactate | pH 8.5, 25°C, isozyme H4 | Sus scrofa | |
0.0057 | - |
(S)-lactate | pH 8.5, 25°C, isozyme H2M2 | Sus scrofa | |
0.0127 | - |
NADH | pH 8.5, 25°C, isozyme H2M2 | Sus scrofa | |
0.0137 | - |
NADH | pH 8.5, 25°C, isozyme H4 | Sus scrofa | |
0.0142 | - |
(S)-lactate | pH 8.5, 25°C, isozyme M4 | Sus scrofa | |
0.0156 | - |
NADH | pH 8.5, 25°C, isozyme M4 | Sus scrofa | |
0.0468 | - |
pyruvate | pH 8.5, 25°C, isozyme H4 | Sus scrofa | |
0.0653 | - |
pyruvate | pH 8.5, 25°C, isozyme H2M2 | Sus scrofa | |
0.13 | - |
pyruvate | pH 8.5, 25°C, isozyme M4 | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | Sus scrofa | - |
pyruvate + NADH + H+ | - |
r | |
pyruvate + NADH + H+ | Sus scrofa | - |
(S)-lactate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P00336 | LDHB | - |
Sus scrofa | P00339 | LDHA | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | contains a skeletal muscle type and heart type mixed heterotetrameric enzyme, H2M2 | Sus scrofa | - |
erythrocyte | - |
Sus scrofa | - |
eye | - |
Sus scrofa | - |
heart | contains a heart type homotetrameric enzyme, H4 | Sus scrofa | - |
kidney | - |
Sus scrofa | - |
liver | - |
Sus scrofa | - |
lung | - |
Sus scrofa | - |
additional information | in somatic cells, LDH forms homotetramers and heterotetramers that are encoded by two different genes: LDHA (skeletal muscle type, M) and LDHB (heart type, H) | Sus scrofa | - |
skeletal muscle | contains a skeletal muscle type homotetrameric enzyme, M4 | Sus scrofa | - |
spleen | - |
Sus scrofa | - |
stomach | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | - |
Sus scrofa | pyruvate + NADH + H+ | - |
r | |
pyruvate + NADH + H+ | - |
Sus scrofa | (S)-lactate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | the heterotetrameric LDH (H2M2) from swine brain is formed by two subunit of LDHA and two subunits of LDHB | Sus scrofa |
More | in somatic cells, LDH forms homotetramers and heterotetramers that are encoded by two different genes: LDHA is the skeletal muscle type, M, isozyme, and LDHB is the heart type, H, isozyme | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
lactate dehydrogenase | - |
Sus scrofa |
LDH | - |
Sus scrofa |
LdhA | skeletal muscle type isozyme | Sus scrofa |
LDHB | heart type isozyme | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sus scrofa | |
NADH | - |
Sus scrofa |
General Information | Comment | Organism |
---|---|---|
metabolism | lactate dehydrogenase (LDH) is a glycolytic enzyme that catalyzes the final step of glycolysis and produces NAD+ | Sus scrofa |