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Literature summary for 1.1.1.27 extracted from

  • Toyoda, K.; Teramoto, H.; Inui, M.; Yukawa, H.
    The ldhA gene, encoding fermentative L-lactate dehydrogenase of Corynebacterium glutamicum, is under the control of positive feedback regulation mediated by LldR (2009), J. Bacteriol., 191, 4251-4258.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene ldhA Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
additional information transposon mutagenesis of a reporter strain carrying a chromosomal ldhA promoter-lacZ fusion, PldhA-lacZ, using Tn5-based minitransposon system reveals that ldhA disruption drastically decreases expression of PldhA-lacZ. ldhA Promoter activity is reduced in the ldhA mutant. PldhA-lacZ expression in the ldhA mutant is restored by deletion of lldR, suggesting that LldR acts as a repressor of ldhA in the absence of L-lactate and the LldR-mediated repression is not relieved in the ldhA mutant due to its inability to produce L-lactate. lldR deletion does not affect PldhA-lacZ expression in the wild-type background during growth on either glucose, acetate, or L-lactate. However, it upregulates PldhA-lacZ expression in the sugR mutant background during growth on acetate. The binding sites of LldR and SugR are located around the -35 and -10 regions of the ldhA promoter, respectively Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lactate + NAD+ Corynebacterium glutamicum
-
 pyruvate + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
 gene ldhA encoding L-lactate dehydrogenase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lactate + NAD+
-
 Corynebacterium glutamicum pyruvate + NADH
-
 r

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Corynebacterium glutamicum
NADH
-
 Corynebacterium glutamicum

Expression

Organism Comment Expression
Corynebacterium glutamicum in the absence of sugar, SugR binds to the ldhA promoter region, thereby repressing ldhA expression. LldR also binds to the ldhA promoter region, thus negatively regulating ldhA expression. LldR is an L-lactate-responsive transcriptional repressor of L-lactate utilization genes down

General Information

General Information Comment Organism
additional information ldhA expression is primarily repressed by SugR in the absence of sugar. In the presence of sugar, SugR-mediated repression of ldhA is alleviated, and ldhA expression is additionally enhanced by LldR inactivation in response to L-lactate produced by LdhA Corynebacterium glutamicum
physiological function LADH-A is a key enzyme that couples L-lactate production to reoxidation of NADH formed during glycolysis Corynebacterium glutamicum