Cloned (Comment) | Organism |
---|---|
gene ldh-a, DNA and amino acid sequence determination and analysis | Amblyrhynchus cristatus |
gene ldh-a, DNA and amino acid sequence determination and analysis | Iguana iguana |
Protein Variants | Comment | Organism |
---|---|---|
I283V | | site-directed mutagenesis, the mutation switches the nature of the residue from Amblyrhynchus cristatus to that of Iguana iguana, another Galapagos marine iguana, the mutation does not affect enzyme kinetics, overview | Amblyrhynchus cristatus |
T9A | site-directed mutagenesis, the mutation switches the nature of the residue from Amblyrhynchus cristatus to that of Iguana iguana, another Galapagos marine iguana, the mutation does not affect enzyme kinetics, overview | Amblyrhynchus cristatus |
T9A/I283V | site-directed mutagenesis, the mutations switch the nature of the residues from Amblyrhynchus cristatus to those of Iguana iguana, another Galapagos marine iguana, the mutation affects enzyme kinetics decreasing Km for pyruvate and kcat, overview | Amblyrhynchus cristatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | Amblyrhynchus cristatus | - |
pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | Iguana iguana | - |
pyruvate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amblyrhynchus cristatus | Q0GND9 | Galapagos marine iguana, gene ldh-a | - |
Iguana iguana | Q0GND8 | common iguana | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | muscle isozyme | Amblyrhynchus cristatus | - |
skeletal muscle | muscle isozyme | Iguana iguana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD+ | - |
Amblyrhynchus cristatus | pyruvate + NADH + H+ | - |
r | |
(S)-lactate + NAD+ | - |
Iguana iguana | pyruvate + NADH + H+ | - |
r | |
additional information | amino acid sequence, structure and kinetic comparison of L-LDH with citrate synthase, EC 2.3.3.1, overview | Amblyrhynchus cristatus | ? | - |
? | |
additional information | amino acid sequence, structure and kinetic comparison of L-LDH with citrate synthase, EC 2.3.3.1, overview | Iguana iguana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecular modeling of the three-dimensional enzyme structure in comparison to the enzyme structure from Amblyrhynchus cristatus, overview | Iguana iguana |
More | molecular modeling of the three-dimensional enzyme structure in comparison to the enzyme structure from Iguana iguana, overview | Amblyrhynchus cristatus |
Synonyms | Comment | Organism |
---|---|---|
A4-LDH | - |
Amblyrhynchus cristatus |
A4-LDH | - |
Iguana iguana |
anaerobic lactate dehydrogenase | - |
Amblyrhynchus cristatus |
anaerobic lactate dehydrogenase | - |
Iguana iguana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Amblyrhynchus cristatus |
30 | - |
assay at | Iguana iguana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
14 | 46 | in vivo temperature | Amblyrhynchus cristatus |
14 | 46 | in vivo temperature | Iguana iguana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Amblyrhynchus cristatus |
7 | - |
assay at | Iguana iguana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Amblyrhynchus cristatus | |
NAD+ | - |
Iguana iguana | |
NADH | - |
Amblyrhynchus cristatus | |
NADH | - |
Iguana iguana |