Cloned (Comment) | Organism |
---|---|
gene ldh | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construct an ethanologenic Bacillus subtilis strain, the ldh gene is disrupted in strain WB700 by chromosomal insertion of the Zymomonas mobilis pyruvate decarboxylase gene pdc and the alcohol dehydrogenase II gene adhB under the control of the ldh native promoter leading to production of etahnol and butanediol by the recombinant strain. But cell growth and glucose consumption rates in strain BS35 are reduced by 70 and 65%, respectively, in comparison to the progenitor strain. To eliminate butanediol production, the acetolactate synthase gene alsS is inactivated resulting in BS36, additional expression of transhydrogenase encoded by gene udhA from Escherichia coli allows a partial recovery of the cell growth rate and an early onset of ethanol production resulting in strain BS37, overview | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD(P)+ | Bacillus subtilis | - |
pyruvate + NAD(P)H + H+ | - |
r | |
(S)-lactate + NAD(P)+ | Bacillus subtilis BS35 | - |
pyruvate + NAD(P)H + H+ | - |
r | |
additional information | Bacillus subtilis | Bacillus subtilis fermentation pathways, overview | ? | - |
? | |
additional information | Bacillus subtilis BS35 | Bacillus subtilis fermentation pathways, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene ldh | - |
Bacillus subtilis BS35 | - |
gene ldh | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cofactor specificity in different strains | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactate + NAD(P)+ | - |
Bacillus subtilis | pyruvate + NAD(P)H + H+ | - |
r | |
(S)-lactate + NAD(P)+ | - |
Bacillus subtilis BS35 | pyruvate + NAD(P)H + H+ | - |
r | |
additional information | Bacillus subtilis fermentation pathways, overview | Bacillus subtilis | ? | - |
? | |
additional information | the enzyme also utilizes NADP(H) | Bacillus subtilis | ? | - |
? | |
additional information | Bacillus subtilis fermentation pathways, overview | Bacillus subtilis BS35 | ? | - |
? | |
additional information | the enzyme also utilizes NADP(H) | Bacillus subtilis BS35 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lactate dehydrogenase | - |
Bacillus subtilis |
LDH | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme also utilizes both NAD(H) and NADP(H) | Bacillus subtilis | |
NAD+ | - |
Bacillus subtilis | |
NADH | - |
Bacillus subtilis | |
NADP+ | - |
Bacillus subtilis | |
NADPH | - |
Bacillus subtilis |