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Literature summary for 1.1.1.27 extracted from

  • Kouassi, G.K.; Anantheswaran, R.C.; Knabel, S.J.; Floros, J.D.
    Effect of high-pressure processing on activity and structure of alkaline phosphatase and lactate dehydrogenase in buffer and milk (2007), J. Agric. Food Chem., 55, 9520-9529.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information loss of LDH activity with increasing pressure and time treatment due to the combined effects of denaturation and aggregation, overview Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bos taurus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + NAD+ Bos taurus
-
pyruvate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
milk fat-free milk, or 2% fat milk Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + NAD+
-
Bos taurus pyruvate + NADH + H+
-
r

Subunits

Subunits Comment Organism
More analysis of enzyme structure under high pressure conditions: even the lowest high pressure processing treatment of 206 MPa induces a reduction in LDH activity, and the course of reduction increases with high pressure processing treatment until complete inactivation at 482, 515, and 620 MPa. The structure of LDH shows gradual denaturation after exposure at 206 MPa for 6 min, leading to a random coil structure at both 515 and 620 MPa. The loss of LDH activity with increasing pressure and time treatment was due to the combined effects of denaturation and aggregation, structure analysis by far-ultraviolet circular dichroism spectroscopy and dynamic light scattetering Bos taurus

Synonyms

Synonyms Comment Organism
LDH
-
Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.8
-
assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
beta-NAD+
-
Bos taurus
beta-NADH
-
Bos taurus