General Stability | Organism |
---|---|
addition of cofactor stabilizes all allozymes to urea inactivation | Fundulus heteroclitus |
inactivation by pronase, trypsin and chymotrypsin | Fundulus heteroclitus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phenylglyoxal | - |
Fundulus heteroclitus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33700 | - |
x * 33700, SDS-PAGE | Fundulus heteroclitus |
139000 | - |
gel filtration | Fundulus heteroclitus |
187000 | - |
non-denaturing gel electrophoresis | Fundulus heteroclitus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fundulus heteroclitus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
allozymes: LDH-Ba4, LDH-Ba/Bb and LDH-Bb4 | Fundulus heteroclitus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
419 | - |
- |
Fundulus heteroclitus |
Storage Stability | Organism |
---|---|
4°C, as ammonium sulfate suspension, stable for more than 8 months | Fundulus heteroclitus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + NADH + H+ | - |
Fundulus heteroclitus | (S)-lactate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33700, SDS-PAGE | Fundulus heteroclitus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
20 min, 50% loss of activity, allozyme LDH-Ba4 | Fundulus heteroclitus |
61 | - |
20 min, 50% loss of activity, allozyme LDH-Ba/Bb | Fundulus heteroclitus |
63 | - |
20 min, 50% loss of activity, allozyme LDH-Bb4 | Fundulus heteroclitus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | coenzyme | Fundulus heteroclitus |