Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Xanthobacter autotrophicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(2-methyl-2-hydroxypropylthio)ethanesulfonate | competitive | Xanthobacter autotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
2-oxopropyl-CoM | pH 7.5, 30°C | Xanthobacter autotrophicus | |
0.096 | - |
2-(R)-hydroxypropyl-CoM | pH 7.5, 30°C | Xanthobacter autotrophicus | |
0.22 | - |
2-(S)-hydroxypropyl-CoM | pH 7.5, 30°C | Xanthobacter autotrophicus | |
0.96 | - |
2-(2-hydroxyethylthio)ethanesulfonate | pH 7.5, 30°C | Xanthobacter autotrophicus | |
220 | - |
(R)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
350 | - |
(S)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthobacter autotrophicus | Q56840 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Xanthobacter autotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-butanol + NAD+ | - |
Xanthobacter autotrophicus | 2-butanone + NADH + H+ | - |
r | |
(S)-2-butanol + NAD+ | - |
Xanthobacter autotrophicus | 2-butanone + NADH + H+ | - |
r | |
2-(2-hydroxyethylthio)ethanesulfonate + NAD+ | achiral mimic of both R-hydroxypropyl-CoM and S-hydroxypropyl-CoM, substrate for both the R- and S-HPCDH enzymes with identical Km values | Xanthobacter autotrophicus | 2-(formylmethylthio)ethanesulfonate + NADH + H+ | - |
r | |
2-(R)-hydroxypropyl-CoM + NAD+ | - |
Xanthobacter autotrophicus | 2-oxopropyl-CoM + NADH + H+ | - |
r | |
2-(S)-hydroxypropyl-CoM + NAD+ | oxidation of S-hydroxypropyl-CoM with a kcat that is 402 times less than that for R-hydroxypropyl-CoM | Xanthobacter autotrophicus | 2-oxopropyl-CoM + NADH + H+ | - |
r | |
2-butanone + NADH + H+ | - |
Xanthobacter autotrophicus | (S)-2-butanol + (R)-2-butanol + NAD+ | without additions, 71.9% (S)-enantiomer + 28% (R)-enantiomer, in presence of 1 mM ethansulfonate 92.7% (S)-enantiomer + 7.3% (R)-enantiomer | r | |
2-oxopropyl-CoM + NADH + H+ | - |
Xanthobacter autotrophicus | 2-(R)-hydroxypropyl-CoM + NAD+ | - |
r | |
additional information | R-HPCDH1 can bind either enantiomer of hydroxypropyl-CoM with the CoM moiety oriented properly in the sulfonate-binding pocket consisting of R152 and R196. A high-affinity ternary complex of S-HPC, NAD+ forms, but the misalignment of the hydrogen and hydroxyl groups on C2 relative to NAD+ and the tyrosine general base results in a 403-fold lower turnover rate for the S-enantiomer | Xanthobacter autotrophicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
xecD | - |
Xanthobacter autotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
2-(S)-hydroxypropyl-CoM | pH 7.5, 30°C | Xanthobacter autotrophicus | |
0.55 | - |
2-(2-hydroxyethylthio)ethanesulfonate | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.9 | - |
(R)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
9.3 | - |
(S)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
29 | - |
2-oxopropyl-CoM | pH 7.5, 30°C | Xanthobacter autotrophicus | |
49 | - |
2-(R)-hydroxypropyl-CoM | pH 7.5, 30°C | Xanthobacter autotrophicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
2-(2-methyl-2-hydroxypropylthio)ethanesulfonate | pH 7.5, 30°C | Xanthobacter autotrophicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
2-(S)-hydroxypropyl-CoM | pH 7.5, 30°C | Xanthobacter autotrophicus | |
8.69 | - |
(R)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
26.4 | - |
(S)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
420 | - |
2-oxopropyl-CoM | pH 7.5, 30°C | Xanthobacter autotrophicus | |
500 | - |
2-(R)-hydroxypropyl-CoM | pH 7.5, 30°C | Xanthobacter autotrophicus |