Application | Comment | Organism |
---|---|---|
drug development | the enzyme from Escherichia coli is a valuable target for the development of antimicrobial compounds | Escherichia coli |
drug development | the enzyme from Mycobacterium smegmatis is a valuable target for the development of antimicrobial compounds | Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-[acetyl(methyl)amino]ethyl phosphate | - |
Escherichia coli | |
2-[acetyl(methyl)amino]ethyl phosphate | - |
Mycolicibacterium smegmatis | |
3-(hydroxyamino)-3-oxopropyl phosphate | - |
Escherichia coli | |
3-(hydroxyamino)-3-oxopropyl phosphate | - |
Mycolicibacterium smegmatis | |
3-[hydroxy(methyl)amino]-3-oxopropyl phosphate | - |
Escherichia coli | |
3-[hydroxy(methyl)amino]-3-oxopropyl phosphate | - |
Mycolicibacterium smegmatis | |
fosfoxacin | - |
Escherichia coli | |
fosfoxacin | - |
Mycolicibacterium smegmatis | |
fosmidomycin | a naturally occurring retrohydroxamate phosphonic acid. DXR-NADPH-fosmidomycin complex crystal structure analysis, the phosphonate group of the inhibitor is located in the phosphate binding cleft of the substrate DXP and is bound in a similar fashion as the phosphate group | Escherichia coli | |
fosmidomycin | a naturally occurring retrohydroxamate phosphonic acid. DXR-NADPH-fosmidomycin complex crystal structure analysis, the phosphonate group of the inhibitor is located in the phosphate binding cleft of the substrate DXP and is bound in a similar fashion as the phosphate group | Mycolicibacterium smegmatis | |
FR-900098 | a naturally occurring retrohydroxamate phosphonic acid, isolated from the filtrate of a Pseudomonas fluorescens PK-52 culture | Mycolicibacterium smegmatis | |
FR900098 | a naturally occurring retrohydroxamate phosphonic acid | Escherichia coli | |
additional information | hydroxamate analogues of fosfoxacin, the phosphate homologue of fosmidomycin, as inhibitors of DXR, synthesis and activities, analysis of Escherichia coli strain XL-1 Blue cell growth inhibition, overview | Escherichia coli | |
additional information | hydroxamate analogues of fosfoxacin, the phosphate homologue of fosmidomycin, as inhibitors of DXR, synthesis and activities, overview | Mycolicibacterium smegmatis | |
[4-(hydroxyamino)-4-oxobutyl]phosphonic acid | - |
Escherichia coli | |
[4-(hydroxyamino)-4-oxobutyl]phosphonic acid | - |
Mycolicibacterium smegmatis | |
[4-[hydroxy(methyl)amino]-4-oxobutyl]phosphonic acid | - |
Escherichia coli | |
[4-[hydroxy(methyl)amino]-4-oxobutyl]phosphonic acid | - |
Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli | |
Mg2+ | required | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Escherichia coli | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Mycolicibacterium smegmatis | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Mycolicibacterium smegmatis ATCC 700084 | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P45568 | - |
- |
Mycolicibacterium smegmatis | A0QVH7 | - |
- |
Mycolicibacterium smegmatis ATCC 700084 | A0QVH7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Mycolicibacterium smegmatis | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Mycolicibacterium smegmatis ATCC 700084 | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-deoxyxylulose 5-phosphate reductoisomerase | - |
Escherichia coli |
1-deoxyxylulose 5-phosphate reductoisomerase | - |
Mycolicibacterium smegmatis |
DXR | - |
Escherichia coli |
DXR | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
7.5 | - |
assay at | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADP+ | - |
Mycolicibacterium smegmatis | |
NADPH | - |
Escherichia coli | |
NADPH | - |
Mycolicibacterium smegmatis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000004 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | FR900098 | |
0.000042 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | fosmidomycin | |
0.000046 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | 3-[hydroxy(methyl)amino]-3-oxopropyl phosphate | |
0.000048 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | [4-[hydroxy(methyl)amino]-4-oxobutyl]phosphonic acid | |
0.000077 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | 2-[acetyl(methyl)amino]ethyl phosphate | |
0.00017 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | [4-(hydroxyamino)-4-oxobutyl]phosphonic acid | |
0.000233 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Mycolicibacterium smegmatis | 3-[hydroxy(methyl)amino]-3-oxopropyl phosphate | |
0.000279 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Mycolicibacterium smegmatis | 2-[acetyl(methyl)amino]ethyl phosphate | |
0.00032 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Mycolicibacterium smegmatis | FR-900098 | |
0.000342 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | fosfoxacin | |
0.00041 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Mycolicibacterium smegmatis | [4-[hydroxy(methyl)amino]-4-oxobutyl]phosphonic acid | |
0.00051 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Mycolicibacterium smegmatis | fosmidomycin | |
0.00148 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Mycolicibacterium smegmatis | [4-(hydroxyamino)-4-oxobutyl]phosphonic acid | |
0.0021 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Mycolicibacterium smegmatis | fosfoxacin | |
0.0026 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Escherichia coli | 3-(hydroxyamino)-3-oxopropyl phosphate | |
0.0092 | - |
pH 7.5, 37°C, recombinant His-tagged enzyme | Mycolicibacterium smegmatis | 3-(hydroxyamino)-3-oxopropyl phosphate |
General Information | Comment | Organism |
---|---|---|
physiological function | in many pathogenic bacteria, the enzymes of the 2-C-methyl-D-erythrirol 4-phosphate (MEP) pathway are involved in the biosynthesis of isopentenyl diphosphate and dimethylallyl diphosphate, the two universal and essential units for the biosynthesis of isoprenoids. The enzyme 1-deoxyxylulose 5-phosphate reductoisomerase (DXR) that catalyzes the transformation of 1-deoxy-D-xylulose 5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) via an isomerization and a NADPH-dependent reduction reaction | Escherichia coli |
physiological function | in many pathogenic bacteria, the enzymes of the 2-C-methyl-D-erythrirol 4-phosphate (MEP) pathway are involved in the biosynthesis of isopentenyl diphosphate and dimethylallyl diphosphate, the two universal and essential units for the biosynthesis of isoprenoids. The enzyme 1-deoxyxylulose 5-phosphate reductoisomerase (DXR) that catalyzes the transformation of 1-deoxy-D-xylulose 5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) via an isomerization and a NADPH-dependent reduction reaction | Mycolicibacterium smegmatis |