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Literature summary for 1.1.1.267 extracted from

  • Kholodar, S.A.; Murkin, A.S.
    DXP reductoisomerase: reaction of the substrate in pieces reveals a catalytic role for the nonreacting phosphodianion group (2013), Biochemistry, 52, 2302-2308.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0098
-
NADPH pH 7.5, 25°C, recombinant enzyme, with substrate 1-deoxy-D-xylulose 5-phosphate Mycobacterium tuberculosis
0.115
-
1-deoxy-D-xylulose 5-phosphate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis
53
-
1-deoxy-L-erythrulose pH 7.5, 25°C, recombinant enzyme in absence or presence of phosphite dianion Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Mycobacterium tuberculosis
phosphite dianion activation by phosphite dianion Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Mycobacterium tuberculosis
-
2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mycobacterium tuberculosis

Reaction

Reaction Comment Organism Reaction ID
2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ random ordered mechanism, though binding of NADPH first is preferential at 0.2 mM due to its low Km. Conformational equilibrium between a predominant, inactive open form and a minor, active closed form of the unliganded enzyme, overview Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
Mycobacterium tuberculosis 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?
1-deoxy-L-erythrulose + NADPH + H+ reaction of the truncated substrate 1-deoxy-L-erythrulose in the presence and absence of phosphite dianion, overview Mycobacterium tuberculosis ? + NADP+
-
?

Synonyms

Synonyms Comment Organism
DXP reductoisomerase
-
Mycobacterium tuberculosis
DXR
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00086
-
1-deoxy-L-erythrulose pH 7.5, 25°C, recombinant enzyme in presence of phosphite dianion Mycobacterium tuberculosis
0.00155
-
1-deoxy-L-erythrulose pH 7.5, 25°C, recombinant enzyme in absence of phosphite dianion Mycobacterium tuberculosis
5.25
-
1-deoxy-D-xylulose 5-phosphate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism 1-deoxy-D-xylulose-5-phosphate, DXP, reductoisomerase catalyzes the first committed step in the 2-C-methyl-D-erythritol 4-phosphate pathway involving a skeletal rearrangement of DXP via a retroaldol/aldol process followed by reduction by NADPH to yield 2-C-methyl-D-erythritol 4-phosphate Mycobacterium tuberculosis
additional information role of the nonreacting phosphorylmethyl group in catalysis, overview Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0000292
-
1-deoxy-L-erythrulose pH 7.5, 25°C, recombinant enzyme in absence of phosphite dianion Mycobacterium tuberculosis
0.000163
-
1-deoxy-L-erythrulose pH 7.5, 25°C, recombinant enzyme in presence of phosphite dianion Mycobacterium tuberculosis
46
-
1-deoxy-D-xylulose 5-phosphate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis