Cloned (Comment) | Organism |
---|---|
into vector pQE30, ligation mixture electroporated into Escherichia coli XL1-Blue cells, giving the recombinant strains XL1-pQEispCMyco and M15-pQEispCMyco | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Escherichia coli | P45568 | - |
- |
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2C-methyl-D-erythritol 4-phosphate + 2C-methyl-D-erythritol 4-phosphate + NADP+ | in reaction mixtures containing 0.15-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana, the reversible reaction can be followed over thousands of reaction cycles. No fragment exchange, and the frequency of exchange, if any, is less than 5 p.p.m. per catalytic cycle | Mycobacterium tuberculosis | ? + NADPH | - |
r | |
2C-methyl-D-erythritol 4-phosphate + 2C-methyl-D-erythritol 4-phosphate + NADP+ | in reaction mixtures containing 0.15-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana, the reversible reaction can be followed over thousands of reaction cycles. No fragment exchange, and the frequency of exchange, if any, is less than 5 p.p.m. per catalytic cycle | Escherichia coli | ? + NADPH | - |
r | |
2C-methyl-D-erythritol 4-phosphate + 2C-methyl-D-erythritol 4-phosphate + NADP+ | in reaction mixtures containing 0.150.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana, the reversible reaction can be followed over thousands of reaction cycles. No fragment exchange, and the frequency of exchange, if any, is less than 5 p.p.m. per catalytic cycle | Arabidopsis thaliana | ? + NADPH | - |
r | |
2C-methyl-D-erythritol 4-phosphate + hydroxyacetone + NADP+ | reaction mixtures containing 0.23-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana | Arabidopsis thaliana | ? + NADPH | - |
? | |
2C-methyl-D-erythritol 4-phosphate + hydroxyacetone + NADP+ | reaction mixtures containing 0.23-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana | Mycobacterium tuberculosis | ? + NADPH | - |
? | |
2C-methyl-D-erythritol 4-phosphate + hydroxyacetone + NADP+ | reaction mixtures containing 0.23-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana | Escherichia coli | ? + NADPH | - |
? | |
glycolaldehyde phosphate + hydroxyacetone + NADPH | reaction mixtures containing 0.21-0.34 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana | Arabidopsis thaliana | ? + NADP+ | - |
? | |
glycolaldehyde phosphate + hydroxyacetone + NADPH | reaction mixtures containing 0.21-0.34 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana | Mycobacterium tuberculosis | ? + NADP+ | - |
? | |
glycolaldehyde phosphate + hydroxyacetone + NADPH | reaction mixtures containing 0.21-0.34 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana | Escherichia coli | ? + NADP+ | - |
? | |
additional information | IspC does not catalyze the isomerisation of 1-deoxy-D-xylulose 5-phosphate to give 1-deoxy-L-ribulose 5-phosphate. Active site of IspC is located close to the surface, a flexible loop at the active site (amino acids 206-216) is able to fold into at least three different conformations | Mycobacterium tuberculosis | ? | - |
? | |
additional information | IspC does not catalyze the isomerisation of 1-deoxy-D-xylulose 5-phosphate to give 1-deoxy-L-ribulose 5-phosphate. Active site of IspC is located close to the surface, a flexible loop at the active site (amino acids 206-216) is able to fold into at least three different conformations | Escherichia coli | ? | - |
? | |
additional information | IspC does not catalyze the isomerisation of 1-deoxy-D-xylulose 5-phosphate to give 1-deoxy-L-ribulose 5-phosphate. Active site of IspC is located close to the surface, a flexible loop at the active site (amino acids 206216) is able to fold into at least three different conformations | Arabidopsis thaliana | ? | - |
? | |
additional information | used for the synthesis of [1-13C1]2C-methyl-D-erythritol 4-phosphate and [3-13C1]2C-methyl-D-erythritol 4-phosphate | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-deoxy-D-xylulose-5-phosphate synthase | - |
Bacillus subtilis |
2C-methyl-D-erythritol-4-phosphate synthase | - |
Arabidopsis thaliana |
2C-methyl-D-erythritol-4-phosphate synthase | - |
Mycobacterium tuberculosis |
2C-methyl-D-erythritol-4-phosphate synthase | - |
Escherichia coli |
DXR | - |
Bacillus subtilis |
DXR | - |
Arabidopsis thaliana |
DXR | - |
Mycobacterium tuberculosis |
DXR | - |
Escherichia coli |
IspC | - |
Arabidopsis thaliana |
IspC | - |
Mycobacterium tuberculosis |
IspC | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Arabidopsis thaliana | |
NADP+ | - |
Mycobacterium tuberculosis | |
NADP+ | - |
Escherichia coli | |
NADPH | - |
Arabidopsis thaliana | |
NADPH | - |
Mycobacterium tuberculosis | |
NADPH | - |
Escherichia coli |