Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.267 extracted from

  • Lauw, S.; Illarionova, V.; Bacher, A.; Rohdich, F.; Eisenreich, W.
    Biosynthesis of isoprenoids: studies on the mechanism of 2C-methyl-D-erythritol-4-phosphate synthase (2008), FEBS J., 275, 4060-4073.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into vector pQE30, ligation mixture electroporated into Escherichia coli XL1-Blue cells, giving the recombinant strains XL1-pQEispCMyco and M15-pQEispCMyco Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Bacillus subtilis
-
-
-
Escherichia coli P45568
-
-
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2C-methyl-D-erythritol 4-phosphate + 2C-methyl-D-erythritol 4-phosphate + NADP+ in reaction mixtures containing 0.15-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana, the reversible reaction can be followed over thousands of reaction cycles. No fragment exchange, and the frequency of exchange, if any, is less than 5 p.p.m. per catalytic cycle Mycobacterium tuberculosis ? + NADPH
-
r
2C-methyl-D-erythritol 4-phosphate + 2C-methyl-D-erythritol 4-phosphate + NADP+ in reaction mixtures containing 0.15-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana, the reversible reaction can be followed over thousands of reaction cycles. No fragment exchange, and the frequency of exchange, if any, is less than 5 p.p.m. per catalytic cycle Escherichia coli ? + NADPH
-
r
2C-methyl-D-erythritol 4-phosphate + 2C-methyl-D-erythritol 4-phosphate + NADP+ in reaction mixtures containing 0.15–0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana, the reversible reaction can be followed over thousands of reaction cycles. No fragment exchange, and the frequency of exchange, if any, is less than 5 p.p.m. per catalytic cycle Arabidopsis thaliana ? + NADPH
-
r
2C-methyl-D-erythritol 4-phosphate + hydroxyacetone + NADP+ reaction mixtures containing 0.23-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana Arabidopsis thaliana ? + NADPH
-
?
2C-methyl-D-erythritol 4-phosphate + hydroxyacetone + NADP+ reaction mixtures containing 0.23-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana Mycobacterium tuberculosis ? + NADPH
-
?
2C-methyl-D-erythritol 4-phosphate + hydroxyacetone + NADP+ reaction mixtures containing 0.23-0.25 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana Escherichia coli ? + NADPH
-
?
glycolaldehyde phosphate + hydroxyacetone + NADPH reaction mixtures containing 0.21-0.34 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana Arabidopsis thaliana ? + NADP+
-
?
glycolaldehyde phosphate + hydroxyacetone + NADPH reaction mixtures containing 0.21-0.34 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana Mycobacterium tuberculosis ? + NADP+
-
?
glycolaldehyde phosphate + hydroxyacetone + NADPH reaction mixtures containing 0.21-0.34 mM IspC from Escherichia coli, Mycobacterium tuberculosis or Arabidopsis thaliana Escherichia coli ? + NADP+
-
?
additional information IspC does not catalyze the isomerisation of 1-deoxy-D-xylulose 5-phosphate to give 1-deoxy-L-ribulose 5-phosphate. Active site of IspC is located close to the surface, a flexible loop at the active site (amino acids 206-216) is able to fold into at least three different conformations Mycobacterium tuberculosis ?
-
?
additional information IspC does not catalyze the isomerisation of 1-deoxy-D-xylulose 5-phosphate to give 1-deoxy-L-ribulose 5-phosphate. Active site of IspC is located close to the surface, a flexible loop at the active site (amino acids 206-216) is able to fold into at least three different conformations Escherichia coli ?
-
?
additional information IspC does not catalyze the isomerisation of 1-deoxy-D-xylulose 5-phosphate to give 1-deoxy-L-ribulose 5-phosphate. Active site of IspC is located close to the surface, a flexible loop at the active site (amino acids 206–216) is able to fold into at least three different conformations Arabidopsis thaliana ?
-
?
additional information used for the synthesis of [1-13C1]2C-methyl-D-erythritol 4-phosphate and [3-13C1]2C-methyl-D-erythritol 4-phosphate Bacillus subtilis ?
-
?

Synonyms

Synonyms Comment Organism
1-deoxy-D-xylulose-5-phosphate synthase
-
Bacillus subtilis
2C-methyl-D-erythritol-4-phosphate synthase
-
Arabidopsis thaliana
2C-methyl-D-erythritol-4-phosphate synthase
-
Mycobacterium tuberculosis
2C-methyl-D-erythritol-4-phosphate synthase
-
Escherichia coli
DXR
-
Bacillus subtilis
DXR
-
Arabidopsis thaliana
DXR
-
Mycobacterium tuberculosis
DXR
-
Escherichia coli
IspC
-
Arabidopsis thaliana
IspC
-
Mycobacterium tuberculosis
IspC
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Arabidopsis thaliana
NADP+
-
Mycobacterium tuberculosis
NADP+
-
Escherichia coli
NADPH
-
Arabidopsis thaliana
NADPH
-
Mycobacterium tuberculosis
NADPH
-
Escherichia coli