Cloned (Comment) | Organism |
---|---|
gene ispC, DNA and amino acid sequence determination and analysis, functional expression of the His-tagged enzyme in the pseudomature form without plastid-targeting sequence in recombinant Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fosmidomycin | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NADPH | pH 8.0, 37°C, recombinant pseudomature enzyme | Arabidopsis thaliana | |
0.132 | - |
1-deoxy-D-xylulose 5-phosphate | pH 8.0, 37°C, recombinant pseudomature enzyme | Arabidopsis thaliana | |
0.47 | 1 | NADP+ | pH 8.0, 37°C, recombinant pseudomature enzyme | Arabidopsis thaliana | |
0.972 | - |
2-C-methyl-D-erythritol 4-phosphate | pH 8.0, 37°C, recombinant pseudomature enzyme | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | a divalent cation is absolutely required, optimal at 10 mM, the enzyme prefers Mn2+ or Mg2+ | Arabidopsis thaliana | |
Mn2+ | a divalent cation is absolutely required, the enzyme prefers Mn2+ or Mg2+ | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Arabidopsis thaliana | second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9XFS9 | DXR; gene ispC | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme in the pseudomature form without plastid-targeting sequence 62fold from Escherichia coli | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
purified recombinant pseudomature enzyme, reverse reaction | Arabidopsis thaliana |
5.6 | - |
purified recombinant pseudomature enzyme, forward reaction | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-20°C, purifed recombinant enzyme, 0.1 M Tris-HCl, pH 7.5, with 50 mM KCl, 10% v/v glycerol, and 10 mM DTT, 6 months, 56% remaining activity | Arabidopsis thaliana |
-80°C, purifed recombinant enzyme, 0.1 M Tris-HCl, pH 7.5, with 50 mM KCl, 10% v/v glycerol, and 10 mM DTT, 6 months, no loss of activity | Arabidopsis thaliana |
4°C, purifed recombinant enzyme, 0.1 M Tris-HCl, pH 7.5, with 50 mM KCl, 10% v/v glycerol, and 10 mM DTT, 6 months, 4% remaining activity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NAD(P)H + H+ | the forward reaction is preferred | Arabidopsis thaliana | 2-C-methyl-D-erythritol 4-phosphate + NAD(P)+ | - |
r | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | second step of the deoxyxylulose 5-phosphate/methylerythritol 4-phosphate pathway with end product isopentenylphosphate | Arabidopsis thaliana | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
IspC | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 42 | 90% of maximal activity within this range | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
purifed recombinant enzyme, 24 h, 78% remaining activity | Arabidopsis thaliana |
37 | - |
purifed recombinant enzyme, 24 h, 30% remaining activity | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
NADP+ | pH 8.0, 37°C, recombinant pseudomature enzyme | Arabidopsis thaliana | |
1.6 | - |
2-C-methyl-D-erythritol 4-phosphate | pH 8.0, 37°C, recombinant pseudomature enzyme | Arabidopsis thaliana | |
4.4 | - |
NADPH | pH 8.0, 37°C, recombinant pseudomature enzyme | Arabidopsis thaliana | |
4.4 | - |
1-deoxy-D-xylulose 5-phosphate | pH 8.0, 37°C, recombinant pseudomature enzyme | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
maximally stable at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana | |
NADH | 14% of the activity with NADPH | Arabidopsis thaliana | |
NADP+ | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000085 | - |
fosmidomycin | pH 8.0, 37°C, recombinant pseudomature enzyme | Arabidopsis thaliana |