Cloned (Comment) | Organism |
---|---|
overexpression in strain BL21 as His-tagged enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | 1,1,1-trifluoro-1-deoxy-D-xylulose 5-phosphoric acid, 1,1-difluoro-1-deoxy-D-xylulose 5-phosphoric acid, and 1,2-dideoxy-D-hexulose 6-phosphate are poor inhibitors, most likely because of the increase in steric bulk at C1 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state and single-turnover kinetics, primary kinetic isotope effects, overview | Escherichia coli | |
0.0005 | - |
NADPH | pH 7.6, 37°C, recombinant enzyme | Escherichia coli | |
0.045 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.6, 37°C, recombinant enzyme | Escherichia coli | |
0.227 | - |
1-fluoro-1-deoxy-D-xylulose 5-phosphate | pH 7.6, 37°C, recombinant enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | Escherichia coli | - |
2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from overexpressing strain BL21 by nickel affinity chromatography | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | the reaction does not exhibit burst kinetics for either substrate, indicating that product release is not rate-limiting, the rearrangement step, which precedes hydride transfer, is rate-limiting for 1-deoxy-D-xylulose 5-phosphate but becomes partially ratelimiting for 1-fluoro-1-deoxy-D-xylulose 5-phosphate, thus, rearrangement appears to be enhanced by substitution of a hydrogen atom in the methyl group of 1-deoxy-D-xylulose 5-phosphate by fluorine, consistent with a retro-aldol/aldol mechanism for the rearrangement during conversion of 1-deoxy-D-xylulose 5-phosphate to 2-C-methyl-D-erythritol 4-phosphate | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | - |
Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
r | |
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ | i.e. DXP | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate + NADP+ | - |
r | |
1-fluoro-1-deoxy-D-xylulose 5-phosphate + NADPH | i.e. CF-DXP, results in about 25% of the activity with 1-deoxy-D-xylulose 5-phosphate | Escherichia coli | ? | - |
r | |
additional information | 1,1,1-trifluoro-1-deoxy-D-xylulose 5-phosphoric acid, 1,1-difluoro-1-deoxy-D-xylulose 5-phosphoric acid, and 1,2-dideoxy-D-hexulose 6-phosphate are poor substrates | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-C-methyl-D-erythritol 4-phosphate synthase | - |
Escherichia coli |
MEP synthase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29 | - |
1-deoxy-D-xylulose 5-phosphate | pH 7.6, 37°C, recombinant enzyme | Escherichia coli | |
37 | - |
1-fluoro-1-deoxy-D-xylulose 5-phosphate | pH 7.6, 37°C, recombinant enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |