Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.267 extracted from

  • Fernandes, R.P.; Phaosiri, C.; Proteau, P.J.
    Mutation in the flexible loop of 1-deoxy-D-xylulose 5-phosphate reductoisomerase broadens substrate utilization (2005), Arch. Biochem. Biophys., 444, 159-164.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
W204A site-directed mutagenesis, the mutant shows reduced activity and altered kinetics compared to the wild-type enzyme Synechocystis sp.
W204F site-directed mutagenesis, the mutant shows reduced activity and altered kinetics compared to the wild-type enzyme, the mutant is active with the substrate analogue 1,2-dideoxy-D-threo-3-hexulose 6-phosphate in contrast to the wild-type enzyme Synechocystis sp.
W204L site-directed mutagenesis, the mutant shows reduced activity and altered kinetics compared to the wild-type enzyme Synechocystis sp.
W204V site-directed mutagenesis, the mutant shows reduced activity and altered kinetics compared to the wild-type enzyme Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
1,2-dideoxy-D-threo-3-hexulose 6-phosphate weak, competitive inhibition, a highly conserved tryptophan residue in the flexible loop is blocked by the substrate analog Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant wild-type enzyme Synechocystis sp.
0.42
-
1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204F Synechocystis sp.
1.3
-
1,2-dideoxy-D-threo-3-hexulose 6-phosphate pH 7.8, 37°C, recombinant mutant W204F Synechocystis sp.
2.9
-
1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204L Synechocystis sp.
5
-
1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204V Synechocystis sp.
12
-
1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204A Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Synechocystis sp.
-
2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
strain PCC6803
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by metal ion affinity chromatography Synechocystis sp.

Reaction

Reaction Comment Organism Reaction ID
2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ the conserved active site residue Trp204 is important in catalysis Synechocystis sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dideoxy-D-threo-3-hexulose 6-phosphate + NADPH + H+ i.e. 1-methyl-1-deoxy-D-xylulose 5-phosphate or M-DXP, a substrate analogue whichis inactive with the wild-type but active with mutant W204F Synechocystis sp. 2-C-ethyl-D-erythritol 4-phosphate + NADP+
-
?
1-deoxy-D-xylulose 5-phosphate + NADPH + H+
-
Synechocystis sp. 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?
1-deoxy-D-xylulose 5-phosphate + NADPH + H+ i.e. DXP Synechocystis sp. 2-C-methyl-D-erythritol 4-phosphate + NADP+
-
?
additional information substrate synthesis and product identification method, overview Synechocystis sp. ?
-
?

Synonyms

Synonyms Comment Organism
DXP reductoisomerase
-
Synechocystis sp.
DXR
-
Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Synechocystis sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
1,2-dideoxy-D-threo-3-hexulose 6-phosphate pH 7.8, 37°C, recombinant mutant W204F Synechocystis sp.
0.16
-
1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204A Synechocystis sp.
0.26
-
1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204L Synechocystis sp.
0.54
-
1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204V Synechocystis sp.
10
-
1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant mutant W204F Synechocystis sp.
17
-
1-deoxy-D-xylulose 5-phosphate pH 7.8, 37°C, recombinant wild-type enzyme Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Synechocystis sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.63
-
1,2-dideoxy-D-threo-3-hexulose 6-phosphate pH 7.8, 37°C, recombinant wild-type enzyme Synechocystis sp.