Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.267 extracted from

  • Hoeffler, J.F.; Tritsch, D.; Grosdemange-Billiard, C.; Rohmer, M.
    Isoprenoid biosynthesis via the methylerythritol phosphate pathway. Mechanistic investigations of the 1-deoxy-D-xylulose 5-phosphate reductoisomerase (2002), Eur. J. Biochem., 269, 4446-4457.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.073
-
1-deoxy-D-xylulose-5-phosphate pH 7.7, presence of 1 mM MnCl2 Escherichia coli
0.097
-
1-deoxy-D-xylulose-5-phosphate pH 7.7, presence of 3 mM MgCl2 Escherichia coli
0.158
-
methyl-D-erythritol-4-phosphate pH 7.7, presence of 3 mM MgCl2 Escherichia coli
0.294
-
methyl-D-erythritol-4-phosphate pH 7.7, presence of 1 mM MnCl2 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ or Mn2+, required Escherichia coli
Mn2+ or Mg2+, required Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
recombinant enzyme, His-tagged
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-deoxy-D-xylulose-5-phosphate + NADPH
-
Escherichia coli methyl-D-erythritol-4-phosphate + NADP+
-
r
additional information reaction depends on NADPH although it does not involve a reduction step, putative aldehyde intermediate is converted to methyl-D-erythritol-4-phosphate in the presence of NADPH and to 1-deoxy-D-xylolose-5-phosphate in the presence of NADP+ Escherichia coli ?
-
?

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Escherichia coli
NADPH
-
Escherichia coli