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Literature summary for 1.1.1.263 extracted from

  • Sakuma, M.; Kubota, S.
    Mouse AKR1E1 is an ortholog of pig liver NADPH dependent 1,5-anhydro-D-fructose reductase (2008), Biosci. Biotechnol. Biochem., 72, 872-876.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli strain BL21(DE3) Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.02
-
1,5-Anhydro-D-fructose pH 7.0, 37°C, recombinant enzyme Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, about, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,5-anhydro-D-fructose + NADPH Mus musculus
-
1,5-anhydro-D-glucitol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 17.4fold from Escherichia coli strain BL21(DE3) by anion exchange and adsorption chromatography, and gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.01
-
purified recombinant enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-fructose + NADPH
-
Mus musculus 1,5-anhydro-D-glucitol + NADP+
-
?
1,5-anhydro-D-fructose + NADPH preferred substrate Mus musculus 1,5-anhydro-D-glucitol + NADP+
-
?
2,3-butanedione + NADPH 76.4% of the activity with 1,5-anhydro-D-fructose Mus musculus ?
-
?
9,10-phenanthrenequinone + NADPH 18% of the activity with 1,5-anhydro-D-fructose Mus musculus ?
-
?
additional information substrate specificities of the recombinant enzyme for carbonyl compounds, overview Mus musculus ?
-
?

Subunits

Subunits Comment Organism
? x * 34000, about, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
AKR1E1
-
Mus musculus
More the enzyme belongs to the aldo-keto reductase family 1 Mus musculus
NADPH dependent 1,5-anhydro-D-fructose reductase
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Mus musculus
NADPH dependent on Mus musculus