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Literature summary for 1.1.1.263 extracted from

  • Sakuma, M.; Kametani, S.; Akanuma, H.
    Purification and some properties of a hepatic NADPH-dependent reductase that specifically acts on 1,5-anhydro-D-fructose (1998), J. Biochem., 123, 189-193.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,5-anhydro-D-glucitol 2.3% inhibition at 100 mM Sus scrofa
2-mercaptoethanol 2.3% inhibition at 1 mM Sus scrofa
ascorbic acid 30% inhibition at 1 mM Sus scrofa
Barbital 15% inhibition at 1 mM Sus scrofa
D-glucose 2.6% inhibition at 100 mM Sus scrofa
D-glucose-1-phosphate 25.5% inhibition at 50 mM Sus scrofa
D-Glucose-6-phosphate 2% inhibition at 50 mM Sus scrofa
EDTA 59% inhibition at 10 mM Sus scrofa
iodoacetamide 41% inhibition at 1 mM Sus scrofa
iodoacetic acid 27% inhibition at 1 mM Sus scrofa
p-chloromercuribenzoic acid 79% inhibition at 0.1 mM, partially reversed by 0.1 mM 2-mercaptoethanol Sus scrofa
Phenobarbital 22% inhibition at 1 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
1,5-Anhydro-D-fructose
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
gel filtration Sus scrofa
38000
-
1 * 38000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,5-anhydro-D-fructose + NADPH Sus scrofa best substrate, only poorly active with NADH 1,5-anhydro-D-glucitol + NADP+
-
ir

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-
Sus scrofa
-
porcine
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-
liver higher activity compared to the porcine liver enzyme Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.16
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
4°C, 20 mM sodium phosphate, pH 7.0, one month Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-fructose + NADPH best substrate, only poorly active with NADH Sus scrofa 1,5-anhydro-D-glucitol + NADP+
-
ir
2,3-butanedione + NADPH 50% of the activity compared to the natural substrate Sus scrofa hydroxybutanone + NADP+
-
?
acetaldehyde + NADPH + H+ very low activity Sus scrofa ethanol + NADP+
-
?
formaldehyde + NADPH low activity Sus scrofa methanol + NADP+
-
?
glucosone + NADPH very low activity Sus scrofa ? + NADP+
-
?
glucuronic acid + NADPH low activity Sus scrofa ? + NADP+
-
?
pyridine-3-aldehyde + NADPH good substrate Sus scrofa pyridine-3-ol + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
decreasing activity above Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.7
-
1,5-Anhydro-D-fructose
-
Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Rattus sp.
NADPH can not replaced by NADH Sus scrofa