Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,5-anhydro-D-glucitol | 2.3% inhibition at 100 mM | Sus scrofa | |
2-mercaptoethanol | 2.3% inhibition at 1 mM | Sus scrofa | |
ascorbic acid | 30% inhibition at 1 mM | Sus scrofa | |
Barbital | 15% inhibition at 1 mM | Sus scrofa | |
D-glucose | 2.6% inhibition at 100 mM | Sus scrofa | |
D-glucose-1-phosphate | 25.5% inhibition at 50 mM | Sus scrofa | |
D-Glucose-6-phosphate | 2% inhibition at 50 mM | Sus scrofa | |
EDTA | 59% inhibition at 10 mM | Sus scrofa | |
iodoacetamide | 41% inhibition at 1 mM | Sus scrofa | |
iodoacetic acid | 27% inhibition at 1 mM | Sus scrofa | |
p-chloromercuribenzoic acid | 79% inhibition at 0.1 mM, partially reversed by 0.1 mM 2-mercaptoethanol | Sus scrofa | |
Phenobarbital | 22% inhibition at 1 mM | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
1,5-Anhydro-D-fructose | - |
Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
gel filtration | Sus scrofa |
38000 | - |
1 * 38000, SDS-PAGE | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-fructose + NADPH | Sus scrofa | best substrate, only poorly active with NADH | 1,5-anhydro-D-glucitol + NADP+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Sus scrofa | - |
porcine | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
liver | higher activity compared to the porcine liver enzyme | Rattus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.16 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
4°C, 20 mM sodium phosphate, pH 7.0, one month | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,5-anhydro-D-fructose + NADPH | best substrate, only poorly active with NADH | Sus scrofa | 1,5-anhydro-D-glucitol + NADP+ | - |
ir | |
2,3-butanedione + NADPH | 50% of the activity compared to the natural substrate | Sus scrofa | hydroxybutanone + NADP+ | - |
? | |
acetaldehyde + NADPH + H+ | very low activity | Sus scrofa | ethanol + NADP+ | - |
? | |
formaldehyde + NADPH | low activity | Sus scrofa | methanol + NADP+ | - |
? | |
glucosone + NADPH | very low activity | Sus scrofa | ? + NADP+ | - |
? | |
glucuronic acid + NADPH | low activity | Sus scrofa | ? + NADP+ | - |
? | |
pyridine-3-aldehyde + NADPH | good substrate | Sus scrofa | pyridine-3-ol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
decreasing activity above | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.7 | - |
1,5-Anhydro-D-fructose | - |
Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rattus sp. | |
NADPH | can not replaced by NADH | Sus scrofa |