Literature summary for 1.1.1.250 extracted from

Kallnik, V.; Schulz, C.; Schultz, C.; Schweiger, P.; Deppenmeier, U.
Properties of recombinant Strep-tagged and untagged hyperthermophilic D-arabitol dehydrogenase from Thermotoga maritima (2011), Appl. Microbiol. Biotechnol., 90, 1285-1293.

Search for more literature for 1.1.1.250

Cloned(Commentary)

Cloned (Comment)	Organism
gene tm0297, located in an operon with tm0298 encoding a mannitol dehydrogenase, phylogenetic analysis, expression of the Strep-tagged enzyme in Escherichia coli	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.027	-	NADH	with D-ribulose, pH 7.5, 85°C, recombinant Strep-tagged enzyme	Thermotoga maritima
0.031	-	NADH	with D-ribulose, pH 7.5, 85°C, recombinant detagged enzyme	Thermotoga maritima
0.059	-	NAD+	with D-arabinitol, pH 8.5, 85°C, recombinant detagged enzyme	Thermotoga maritima
0.069	-	NAD+	with D-arabinitol, pH 8.5, 85°C, recombinant Strep-tagged enzyme	Thermotoga maritima
2.6	-	D-arabinitol	pH 8.5, 85°C, recombinant detagged enzyme	Thermotoga maritima
7.1	-	D-arabinitol	pH 8.5, 85°C, recombinant Strep-tagged enzyme	Thermotoga maritima
7.2	-	D-ribulose	pH 7.5, 85°C, recombinant detagged enzyme	Thermotoga maritima
13.9	-	D-ribulose	pH 7.5, 85°C, recombinant Strep-tagged enzyme	Thermotoga maritima
20	-	D-xylitol	pH 8.5, 85°C, recombinant Strep-tagged enzyme	Thermotoga maritima
21.7	-	D-xylitol	pH 8.5, 85°C, recombinant detagged enzyme	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	twofold activation at 60 mM, contributes to the overall stability of the protein and ise not required for catalytic activity	Thermotoga maritima
Mg2+	twofold activation at 55 mM, contributes to the overall stability of the protein and is not required for catalytic activity	Thermotoga maritima
additional information	no effect on the activity by CaCl2 or NaCl	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27600	-	6 * 27600, SDS-PAGE	Thermotoga maritima
164000	-	native PAGE	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	-	gene tm0297	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	Thermotoga maritima cannot use D-arabitol as a sole carbon source. Optimal growth temperature of Thermotoga maritima is 80°C	Thermotoga maritima	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-arabinitol + NAD+	-	Thermotoga maritima	D-ribulose + NADH + H+	-	r
D-xylitol + NAD+	-	Thermotoga maritima	D-xylulose + NADH + H+	-	r
additional information	the enzyme exclusively catalyzed the NAD(H)-dependent oxidoreduction of D-arabitol, D-xylitol, D-ribulose, or D-xylulose	Thermotoga maritima	?	-	?

Subunits

Subunits	Comment	Organism
homohexamer	6 * 27600, SDS-PAGE	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
arabitol 2-dehydrogenase	-	Thermotoga maritima
ArDH	-	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
85	-	-	Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
50	85	inactive below 50°C, optimal activity at 85°C	Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	the addition of the Strep-tag led to a destabilization of tmArDH resulting in the loss of thermostability	Thermotoga maritima
85	-	rapid inactivation of the recombinant Strep-tagged enzyme, while the detagged enzyme retains over 90% activity after 90 min at 85°C	Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
11	-	D-arabinitol	pH 8.5, 85°C, recombinant Strep-tagged enzyme	Thermotoga maritima
11.2	-	D-xylitol	pH 8.5, 85°C, recombinant Strep-tagged enzyme	Thermotoga maritima
16.6	-	D-xylitol	pH 8.5, 85°C, recombinant detagged enzyme	Thermotoga maritima
18.7	-	D-arabinitol	pH 8.5, 85°C, recombinant detagged enzyme	Thermotoga maritima
165.6	-	D-ribulose	pH 7.5, 85°C, recombinant Strep-tagged enzyme	Thermotoga maritima
193.2	-	D-ribulose	pH 7.5, 85°C, recombinant detagged enzyme	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	reduction reaction	Thermotoga maritima
8.5	-	oxidation reaction	Thermotoga maritima

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	9.5	40% and 10% of maximal activity at pH 5.5 and pH 8.5, no activity below pH 4.5 and above pH 9.5	Thermotoga maritima

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	dependent on	Thermotoga maritima
NADH	dependent on	Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.56	-	D-xylitol	pH 8.5, 85°C, recombinant Strep-tagged enzyme	Thermotoga maritima
0.74	-	D-xylitol	pH 8.5, 85°C, recombinant detagged enzyme	Thermotoga maritima
1.5	-	D-arabinitol	pH 8.5, 85°C, recombinant Strep-tagged enzyme	Thermotoga maritima
7.2	-	D-arabinitol	pH 8.5, 85°C, recombinant detagged enzyme	Thermotoga maritima
12	-	D-ribulose	pH 7.5, 85°C, recombinant Strep-tagged enzyme	Thermotoga maritima
27	-	D-ribulose	pH 7.5, 85°C, recombinant detagged enzyme	Thermotoga maritima

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Release 2023.1 (January 2023)