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Literature summary for 1.1.1.25 extracted from

  • Zhang, J.; ten Pierick, A.; van Rossum, H.M.; Seifar, R.M.; Ras, C.; Daran, J.M.; Heijnen, J.J.; Wahl, S.A.
    Determination of the cytosolic NADPH/NADP ratio in Saccharomyces cerevisiae using shikimate dehydrogenase as sensor reaction (2015), Sci. Rep., 5, 12846.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis usage of Escherichia coli shikimate dehydrogenase as sensor reaction for determination of the cytosolic NADPH/NADP ratio in Saccharomyces cerevisiae, quantitative measurements of physiological variables in the cytosolic compartment by GC-MS/MS, cytosolic NADPH/NADP ratio in batch experiments, overview. The steady state sensor reaction based cytosolic free NADPH/NADP ratio is 15.6 Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene aroE from Escherichia coli sstrain DH5alpha, recombinant overexpression in Saccharomyces cerevisiae strain CEN.PK 113-5D yielding strain CEN.PK-aroE Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information steady state cytosolic free and whole cell NADPH/NADP ratio in different Saccharomycs cerevisiae strains, with or without recombinant expression of shikimate dehydrogenase from Escherichia coli, thermodynamics and kinetics, overview Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol occurs only in the cytosol Escherichia coli 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
shikimate + NADP+ Escherichia coli
-
3-dehydroshikimate + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli P15770
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.68
-
recombinant CEN.PK-aroE strain expressing the enzyme from Eschericha coli, pH 7.0, 25°C Escherichia coli
19.9
-
pH 9.0, 25°C, recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information usage of recombinant shikimate dehydrogenase as sensor reaction for determination of the cytosolic NADPH/NADP ratio in Saccharomyces cerevisiae, quantitative measurements of physiological variables in the cytosolic compartment by GC-MS/MS, cytosolic NADPH/NADP ratio in batch experiments, method, overview Escherichia coli ?
-
?
shikimate + NADP+
-
Escherichia coli 3-dehydroshikimate + NADPH + H+
-
r

Synonyms

Synonyms Comment Organism
AroE
-
Escherichia coli
SDH
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli
30
-
in vivo assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
in vivo assay at Escherichia coli
9
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+ specific for Escherichia coli
NADPH specific for Escherichia coli

General Information

General Information Comment Organism
additional information modelling of steady state and dynamic fluxes into pentose phosphate pathway and the flux split ratio into glycolysis and pentose phosphate pathway in Saccharomyces recombinantly expressing Escherichia coli shikimate dehydrogenase, overview Escherichia coli