Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.25 extracted from

  • Kubota, T.; Tanaka, Y.; Hiraga, K.; Inui, M.; Yukawa, H.
    Characterization of shikimate dehydrogenase homologues of Corynebacterium glutamicum (2013), Appl. Microbiol. Biotechnol., 97, 8139-8149.
    View publication on PubMed
Search for more literature for 1.1.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: quinate; not inhibitory: quinate Corynebacterium glutamicum
shikimate shikimate synthesis decreases with increasing shikimate concentration. The relative activity halves at about 1.4 mM shikimate; shikimate synthesis decreases with increasing shikimate concentration. The relative activity halves at about 1.4 mM shikimate Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00036
-
 3-dehydroquinate pH 7.0, 30°C Corynebacterium glutamicum
0.14
-
 shikimate pH 9.0, 30°C Corynebacterium glutamicum
0.187
-
 shikimate pH 7.0, 30°C Corynebacterium glutamicum
0.2
-
 3-dehydroshikimate pH 7.0, 30°C Corynebacterium glutamicum
0.272
-
 3-dehydroshikimate pH 9.0, 30°C Corynebacterium glutamicum
0.42
-
 3-dehydroquinate pH 7.0, 30°C Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum A4QB65
-
-
Corynebacterium glutamicum A4QEK4 putative
-
Corynebacterium glutamicum JCM 18229 A4QB65
-
-
Corynebacterium glutamicum JCM 18229 A4QEK4 putative
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydroquinate + NADH + H+
-
 Corynebacterium glutamicum quinate + NAD+
-
 r
3-dehydroquinate + NADH + H+
-
 Corynebacterium glutamicum JCM 18229 quinate + NAD+
-
 r
3-dehydroquinate + NADPH + H+
-
 Corynebacterium glutamicum quinate + NADP+
-
 r
3-dehydroquinate + NADPH + H+
-
 Corynebacterium glutamicum JCM 18229 quinate + NADP+
-
 r
3-dehydroshikimate + NADH + H+ less than 1% of the rate with 3-dehydroquinate Corynebacterium glutamicum shikimate + NAD+
-
 r
3-dehydroshikimate + NADH + H+ less than 1% of the rate with 3-dehydroquinate Corynebacterium glutamicum JCM 18229 shikimate + NAD+
-
 r
3-dehydroshikimate + NADPH + H+
-
 Corynebacterium glutamicum shikimate + NADP+
-
 r
3-dehydroshikimate + NADPH + H+
-
 Corynebacterium glutamicum JCM 18229 shikimate + NADP+
-
 r
quinate + NAD+
-
 Corynebacterium glutamicum 3-dehydroquinate + NADH + H+
-
 r
quinate + NAD+
-
 Corynebacterium glutamicum JCM 18229 3-dehydroquinate + NADH + H+
-
 r
shikimate + NADP+
-
 Corynebacterium glutamicum 3-dehydroshikimate + NADPH + H+
-
 r
shikimate + NADP+
-
 Corynebacterium glutamicum JCM 18229 3-dehydroshikimate + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
AroE
-
 Corynebacterium glutamicum
cgR_0495
-
 Corynebacterium glutamicum
cgR_1677
-
 Corynebacterium glutamicum
qsuD
-
 Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.36
-
 3-dehydroquinate pH 7.0, 30°C Corynebacterium glutamicum
31
-
 shikimate pH 7.0, 30°C Corynebacterium glutamicum
114
-
 3-dehydroquinate pH 7.0, 30°C Corynebacterium glutamicum
118
-
 3-dehydroshikimate pH 9.0, 30°C Corynebacterium glutamicum
234
-
 shikimate pH 9.0, 30°C Corynebacterium glutamicum
329
-
 3-dehydroshikimate pH 7.0, 30°C Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 reduction of 3-dehydroquinate Corynebacterium glutamicum
7
-
 reduction of 3-dehydroshikimate Corynebacterium glutamicum
8.5
-
 oxidation of quinate Corynebacterium glutamicum
9
-
 oxidation of shikimate Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
additional information NADH shows about 2% of the activity with NADPH Corynebacterium glutamicum
additional information NADPH shows about 2.5% of the activity with NADPH Corynebacterium glutamicum
NAD+
-
 Corynebacterium glutamicum
NADH
-
 Corynebacterium glutamicum
NADP+
-
 Corynebacterium glutamicum
NADPH
-
 Corynebacterium glutamicum

Expression

Organism Comment Expression
Corynebacterium glutamicum isoform qsuD mRNA expression in shikimate-grown cells is upregulated relative to that in the glucose-grown cells up

General Information

General Information Comment Organism
physiological function a strain deficient in isoform qsuD does not grow on either shikimate or quinate as sole carbon sources but grows largely unhindered on glucose Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.143
-
 3-dehydroquinate pH 7.0, 30°C Corynebacterium glutamicum
168
-
 shikimate pH 7.0, 30°C Corynebacterium glutamicum
272
-
 3-dehydroquinate pH 7.0, 30°C Corynebacterium glutamicum
435
-
 3-dehydroshikimate pH 9.0, 30°C Corynebacterium glutamicum
1650
-
 3-dehydroshikimate pH 7.0, 30°C Corynebacterium glutamicum
1670
-
 shikimate pH 9.0, 30°C Corynebacterium glutamicum