Crystallization (Comment) | Organism |
---|---|
diffraction to 1.6 A | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.2 | - |
quinate | pH 9.0, 30°C | Corynebacterium glutamicum | |
46.6 | - |
shikimate | pH 10.0, 30°C | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q9X5C9 | quinate/shikimate dehydrogenase | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+ | active-site base is K92. After the binding of quinate and NAD+, the oxygen of the C3-OH of quinate forms a hydrogen bond to the side chain of the conserved T88 and K92 functions as the active-site base to remove the proton on the C3-OH. Simultaneously,a hydride is transferred from C3 of quinate to NAD+ | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinate + NAD+ | in contrast to shikimate, quinate may form a hydrogen bond to the NAD+, and the hydroxyl group of a active-site threonine hydrogen bonds to quinate more effectively than shikimate resulting in a lower Michaelis constant and higher catalytic efficiency for quinate | Corynebacterium glutamicum | 3-dehydroquinate + NADH + H+ | - |
? | |
shikimate + NAD+ | - |
Corynebacterium glutamicum | 3-dehydroshikimate + NADH + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
61.9 | - |
quinate | pH 9.0, 30°C | Corynebacterium glutamicum | |
85.2 | - |
shikimate | pH 10.0, 30°C | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | preferred over NADP+ | Corynebacterium glutamicum | |
NADP+ | turnover number decreases by more than 300fold when NADP+ is used instead of NAD+, regardless of substrate shikimate or quinate | Corynebacterium glutamicum |