Cloned (Comment) | Organism |
---|---|
gene aroE, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-([2-([2-([2-(2,3-dimethylanilino)-2-oxoethyl]sulfanyl)-1,3-benzothiazol-6-yl]amino)2-oxoethyl]sulfanyl)-N-(2-naphthyl)acetamide | IC50: 0.0029 mM, competitive inhibition with respect to shikimate, noncompetitive to NADP+, potent antibacterial activity | Helicobacter pylori | |
3-(2-naphthyloxy)-4-oxo-2-(trifluoromethyl)-4H-chromen-7-yl 3-chlorobenzoate | IC50: 0.0039 mM, noncompetitive inhibition with respect to shikimate, competitive to NADP+ | Helicobacter pylori | |
butyl 2-([3-(2-naphthyloxy)4-oxo-2-(trifluoromethyl)4H-chromen-7-yl]oxy)propanoate | IC50: 0.0134 mM, noncompetitive inhibition with respect to shikimate, competitive to NADP+, potent antibacterial activity | Helicobacter pylori | |
curcumin | IC50: 0.0154 mM, noncompetitive inhibition with respect to shikimate and NADP+ | Helicobacter pylori | |
maesaquinone diacetate | IC50: 0.0035 mM, noncompetitive inhibition with respect to shikimate and NADP+ | Helicobacter pylori | |
additional information | antibacterial activity of inhibitors, overview | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.148 | - |
shikimate | pH 8.0, 60°C, recombinant enzyme | Helicobacter pylori | |
0.182 | - |
NADP+ | pH 8.0, 60°C, recombinant enzyme | Helicobacter pylori | |
2.9 | - |
NAD+ | pH 8.0, 60°C, recombinant enzyme | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydroshikimate + NAD(P)H + H+ | Helicobacter pylori | fourth enzyme involved in the shikimate pathway | shikimate + NAD(P)+ | - |
r | |
3-dehydroshikimate + NAD(P)H + H+ | Helicobacter pylori SS1 | fourth enzyme involved in the shikimate pathway | shikimate + NAD(P)+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | Q56S04 | gene aroE; gene aroE | - |
Helicobacter pylori SS1 | Q56S04 | gene aroE; gene aroE | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Helicobacter pylori |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-dehydroshikimate + NAD(P)H + H+ | - |
Helicobacter pylori | shikimate + NAD(P)+ | - |
r | |
3-dehydroshikimate + NAD(P)H + H+ | fourth enzyme involved in the shikimate pathway | Helicobacter pylori | shikimate + NAD(P)+ | - |
r | |
3-dehydroshikimate + NAD(P)H + H+ | - |
Helicobacter pylori SS1 | shikimate + NAD(P)+ | - |
r | |
3-dehydroshikimate + NAD(P)H + H+ | fourth enzyme involved in the shikimate pathway | Helicobacter pylori SS1 | shikimate + NAD(P)+ | - |
r | |
quinate + NADP+ | - |
Helicobacter pylori | 3-dehydroquinate + NADPH + H+ | - |
r | |
quinate + NADP+ | - |
Helicobacter pylori SS1 | 3-dehydroquinate + NADPH + H+ | - |
r | |
shikimate + NAD+ | - |
Helicobacter pylori | 3-dehydroshikimate + NADH + H+ | - |
r | |
shikimate + NAD+ | - |
Helicobacter pylori SS1 | 3-dehydroshikimate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | analysis of secondary structure | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
SDH | - |
Helicobacter pylori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
oxidation reaction, recombinant His-tagged enzyme | Helicobacter pylori |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | - |
Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
NAD+ | pH 8.0, 60°C, recombinant enzyme | Helicobacter pylori | |
7.1 | - |
NADP+ | pH 8.0, 60°C, recombinant enzyme | Helicobacter pylori | |
7.7 | - |
shikimate | pH 8.0, 60°C, recombinant enzyme | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | oxidation reaction, recombinant His-tagged enzyme | Helicobacter pylori |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profile of the oxidation reaction | Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Helicobacter pylori | |
NADH | - |
Helicobacter pylori | |
NADP+ | preferred cofactor | Helicobacter pylori | |
NADPH | preferred cofactor | Helicobacter pylori |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0029 | - |
IC50: 0.0029 mM, competitive inhibition with respect to shikimate, noncompetitive to NADP+, potent antibacterial activity | Helicobacter pylori | 2-([2-([2-([2-(2,3-dimethylanilino)-2-oxoethyl]sulfanyl)-1,3-benzothiazol-6-yl]amino)2-oxoethyl]sulfanyl)-N-(2-naphthyl)acetamide | |
0.0035 | - |
IC50: 0.0035 mM, noncompetitive inhibition with respect to shikimate and NADP+ | Helicobacter pylori | maesaquinone diacetate | |
0.0039 | - |
IC50: 0.0039 mM, noncompetitive inhibition with respect to shikimate, competitive to NADP+ | Helicobacter pylori | 3-(2-naphthyloxy)-4-oxo-2-(trifluoromethyl)-4H-chromen-7-yl 3-chlorobenzoate | |
0.0134 | - |
IC50: 0.0134 mM, noncompetitive inhibition with respect to shikimate, competitive to NADP+, potent antibacterial activity | Helicobacter pylori | butyl 2-([3-(2-naphthyloxy)4-oxo-2-(trifluoromethyl)4H-chromen-7-yl]oxy)propanoate | |
0.0154 | - |
IC50: 0.0154 mM, noncompetitive inhibition with respect to shikimate and NADP+ | Helicobacter pylori | curcumin |