Literature summary for 1.1.1.25 extracted from

Schoepe, J.; Niefind, K.; Chatterjee, S.; Schomburg, D.
Cloning, expression, purification and preliminary crystallographic characterization of a shikimate dehydrogenase from Corynebacterium glutamicum (2006), Acta Crystallogr. Sect. F, F62, 635-637.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	Corynebacterium glutamicum

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, sitting drop vapour diffusion method, 3.75 mg/ml protein in 50 mM Tris-HCl, pH 7.5, 0.5 M NaCl, 1 mM NaN3, and 20% v/v glycerol, the reservoir solution contains 0.1 M sodium acetate, pH 4.6, 0.2 M NaCl, and 20% v/v precipitant 2,4-methylenepentanediol, 0.002 ml of protein, reservoir and 0.002 mM NADH solutions are mixed at 20°C, about 5 months, formation of rod- and needle-shaped crystals, X-ray diffraction structure determination and analysis at 1.64 A resolution	Corynebacterium glutamicum

Crystallization (Comment)

Organism

purified recombinant enzyme, sitting drop vapour diffusion method, 3.75 mg/ml protein in 50 mM Tris-HCl, pH 7.5, 0.5 M NaCl, 1 mM NaN3, and 20% v/v glycerol, the reservoir solution contains 0.1 M sodium acetate, pH 4.6, 0.2 M NaCl, and 20% v/v precipitant 2,4-methylenepentanediol, 0.002 ml of protein, reservoir and 0.002 mM NADH solutions are mixed at 20°C, about 5 months, formation of rod- and needle-shaped crystals, X-ray diffraction structure determination and analysis at 1.64 A resolution

Corynebacterium glutamicum

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	-	strain ATCC 13032	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)	Corynebacterium glutamicum

Synonyms

Synonyms	Comment	Organism
SDH	-	Corynebacterium glutamicum

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Release 2023.1 (January 2023)