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Literature summary for 1.1.1.25 extracted from
- Shikimate dehydrogenase from pepper (Capsicum annuum) seedlings. Purification and properties (1997), Physiol. Plant., 100, 147-152.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Capsicum annuum |  |
| Mg2+ | - |
Capsicum annuum | |
| Mn2+ | - |
Capsicum annuum | |
| NH4+ | - |
Capsicum annuum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-Dichlorophenoxyacetic acid | - |
Capsicum annuum | |
| Cu2+ | - |
Capsicum annuum | |
| guaiacol | - |
Capsicum annuum | |
| protocatechuic acid | competitive inhibition | Capsicum annuum | |
| Zn2+ | - |
Capsicum annuum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
NADP+ | - |
Capsicum annuum | |
| 0.087 | - |
shikimic acid | NADP+ | Capsicum annuum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
gel filtration, 4 different isoenzymes | Capsicum annuum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-dehydroshikimate + NADPH | Capsicum annuum | - |
shikimate + NADP+ | - |
r | |
| shikimate + NADP+ | Capsicum annuum | - |
3-dehydroshikimate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Capsicum annuum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| separation of isoenzymes by regaining the protein from PA-gel slices | Capsicum annuum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-dehydroshikimate + NADPH | - |
Capsicum annuum | shikimate + NADP+ | - |
r | |
| shikimate + NADP+ | - |
Capsicum annuum | 3-dehydroshikimate + NADPH + H+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Capsicum annuum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
at temperatures higher than 40°C the enzyme is quickly inactivated | Capsicum annuum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
- |
Capsicum annuum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Capsicum annuum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | stable for 45 min | Capsicum annuum |
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