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Literature summary for 1.1.1.25 extracted from

  • Maclean, J.; Campbell, S.A.; Pollock, K.; Chackrewarthy, S.; Coggins, J.R.; Lapthorn, A.J.
    Crystallization and preliminary X-ray analysis of shikimate dehydrogenase from Escherichia coli (2000), Acta Crystallogr. Sect. D, 56, 512-515.
    View publication on PubMed
Search for more literature for 1.1.1.25

Cloned(Commentary)

Cloned (Comment) Organism
gene aroE, overexpression in the aroE auxotrophic mutant strain AB2834 Escherichia coli
overexpression in Escherichia coli K12 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli
purified recombinant enzyme, 20 mg/ml in 10 mM Tris-HCl, pH 7.6, 0.4 mM DTT, and 2.5 mM NADPH, 20°C, sitting-drop vapour-diffusion method, reservoir solution contains 1.65 M ammonium sulfate, 100 mM cacodylate buffer, pH 5.8, 1.5 ml of protein solution is mixed with an equal volume of reservoir solution, 10-12 days, cryoprotection with 20% v/v glycerol, X-ray diffraction structure determination and analysis at 2.3 A resolution Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29400
-
 mass spectometry and calculation from DNA sequence Escherichia coli
29410
-
 mass spectrometry Escherichia coli
29414
-
 1 * 29414, mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
shikimate + NADP+ Escherichia coli
-
 3-dehydroshikimate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli
recombinant enzyme from aroE auxotrophic mutant strain AB2834 by ammonium sulfate fractionation, anion exchange chromatography, ultrafiltration, and gel filtration Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, Tris-HCl, pH 7.5, 0.4 mM DTT, 50% glycerol, long-term Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
shikimate + NADP+
-
 Escherichia coli 3-dehydroshikimate + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29400, mass spectrometry and gel filtration Escherichia coli
monomer 1 * 29414, mass spectrometry Escherichia coli
More structure analysis, dynamic light scattering measurements Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Escherichia coli
NADPH
-
 Escherichia coli