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Literature summary for 1.1.1.248 extracted from

  • Ziegler, J.; Brandt, W.; Geissler, R.; Facchini, P.J.
    Removal of substrate inhibition and increase in maximal velocity in the short chain dehydrogenase/reductase salutaridine reductase involved in morphine biosynthesis (2009), J. Biol. Chem., 284, 26758-26767.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.248

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli SG13009 Papaver somniferum

Protein Variants

Protein Variants Comment Organism
D107A increase in Km, 2fold decrease in kcat Papaver somniferum
F104A increase in kcat Papaver somniferum
F104A/I275A shows no substrate inhibition accompanied by a weak affinity for productive salutaridine binding, kcat is almost 2fold higher compared with the wild-type Papaver somniferum
I275A increase in Km, shows an increase of velocity by a factor of 2.3 Papaver somniferum
K186V moderate effects on substrate affinity, exhibits a turnover number similar to that of the wild-type Papaver somniferum
L185A increase in Km, decrease in kcat Papaver somniferum
L266A decrease in kcat Papaver somniferum
M271A decrease in kcat Papaver somniferum
M271A increase in Km Papaver somniferum
N272A increase in Km, decrease in kcat Papaver somniferum
S181A moderate effects on substrate affinity, increase in kcat by 50% Papaver somniferum
T182A moderate effects on substrate affinity Papaver somniferum
V106A moderate effects on substrate affinity, increase in kcat by 90% Papaver somniferum

Inhibitors

Inhibitors Comment Organism Structure
additional information strong substrate inhibition of SalR Papaver somniferum
NADP+ product inhibition Papaver somniferum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 salutaridine wild-type Papaver somniferum
0.0034
-
 NADPH mutant K186V Papaver somniferum
0.0035
-
 NADPH wild-type Papaver somniferum
0.0041
-
 salutaridine mutant T182A Papaver somniferum
0.0045
-
 NADPH mutant S181A Papaver somniferum
0.005
-
 salutaridine mutant S181A Papaver somniferum
0.007
-
 NADPH mutant V106A Papaver somniferum
0.0078
-
 salutaridine mutant K186V Papaver somniferum
0.0089
-
 salutaridine mutant V106A Papaver somniferum
0.0097
-
 NADPH mutant F104A Papaver somniferum
0.0132
-
 salutaridine mutant L185A Papaver somniferum
0.0148
-
 salutaridine mutant D107A Papaver somniferum
0.0155
-
 NADPH mutant L266A Papaver somniferum
0.0156
-
 NADPH mutant N272A Papaver somniferum
0.0198
-
 NADPH mutant M271A Papaver somniferum
0.0211
-
 NADPH mutant I275A Papaver somniferum
0.0213
-
 NADPH mutant T182A Papaver somniferum
0.0225
-
 salutaridine mutant M271A Papaver somniferum
0.0297
-
 NADPH mutant L185A Papaver somniferum
0.0305
-
 NADPH mutant D107A Papaver somniferum
0.0318
-
 salutaridine mutant F104A Papaver somniferum
0.0331
-
 salutaridine mutant I275A Papaver somniferum
0.043
-
 NADPH mutant F104A/I275A Papaver somniferum
0.0494
-
 salutaridine mutant L266A Papaver somniferum
0.0901
-
 salutaridine mutant N272A Papaver somniferum
0.4086
-
 salutaridine mutant F104A/I275A Papaver somniferum

Organism

Organism UniProt Comment Textmining
Papaver somniferum Q071N0
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Papaver somniferum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
salutaridine + NADPH + H+
-
 Papaver somniferum (7S)-salutaridinol + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
SalR
-
 Papaver somniferum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
 salutaridine mutant M271A Papaver somniferum
0.016
-
 salutaridine mutant L185A Papaver somniferum
0.021
-
 salutaridine mutant N272A Papaver somniferum
0.126
-
 salutaridine mutant T182A Papaver somniferum
0.501
-
 salutaridine mutant L266A Papaver somniferum
0.61
-
 salutaridine mutant D107A Papaver somniferum
0.998
-
 salutaridine mutant K186V Papaver somniferum
1.306
-
 salutaridine wild-type Papaver somniferum
1.933
-
 salutaridine mutant S181A Papaver somniferum
2.45
-
 salutaridine mutant F104A/I275A Papaver somniferum
2.458
-
 salutaridine mutant V106A Papaver somniferum
2.727
-
 salutaridine mutant F104A Papaver somniferum
3.188
-
 salutaridine mutant I275A Papaver somniferum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Papaver somniferum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0012
-
 NADP+
-
 Papaver somniferum