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Literature summary for 1.1.1.247 extracted from

  • Sharafi, A.; Sohi, H.H.; Mousavi, A.; Azadi, P.; Khalifani, B.H.; Razavi, K.
    Metabolic engineering of morphinan alkaloids by over-expression of codeinone reductase in transgenic hairy roots of Papaver bracteatum, the Iranian poppy (2013), Biotechnol. Lett., 35, 445-453.
    View publication on PubMed

Application

Application Comment Organism
synthesis Papaver bracteatum hairy roots expressing CodR gene have a high potential to produce morphinan alkaloids Papaver somniferum

Cloned(Commentary)

Cloned (Comment) Organism
gene codR, expression in Papaver bracteatum in cultured hairy roots, subcloning in Escherichia coli strain DH5alpha. The T-DNA of the vector contains a neomycin phosphoteransferase gene and CodR cDNA with CaMV35S promoter and nos terminator Papaver somniferum

Protein Variants

Protein Variants Comment Organism
additional information transgenic codeinone reductase overexpressing Papaver somniferum plants show 75% increase in thebaine besides 58% and 22% increases in codeine and morphine, respectively, compared to the wild-type plants in the field trail Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
codeinone + NADPH + H+ Papaver somniferum
-
codeine + NADP+
-
r
additional information Papaver somniferum the enzyme also catalyzes the NADPH-dependent reduction of morphinone to morphine, reaction of EC 1.1.1.218. but codeine can also be directly converted into morphine, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Papaver somniferum Q9SQ67
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Papaver somniferum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
codeinone + NADPH + H+
-
Papaver somniferum codeine + NADP+
-
r
additional information the enzyme also catalyzes the NADPH-dependent reduction of morphinone to morphine, reaction of EC 1.1.1.218. but codeine can also be directly converted into morphine, overview Papaver somniferum ?
-
?

Synonyms

Synonyms Comment Organism
codeinone reductase (NADPH)
-
Papaver somniferum
CodR
-
Papaver somniferum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Papaver somniferum
NADPH
-
Papaver somniferum