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Literature summary for 1.1.1.245 extracted from

  • Dangel, W.; Tschech, A.; Fuchs, G.
    Enzyme reactions involved in anaerobic cyclohexanol metabolism by a denitrifying Pseudomonas species (1989), Arch. Microbiol., 152, 273-279.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
NAD+
-
Pseudomonas sp.
0.05
-
Cyclohexanol
-
Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cyclohexanol + NAD+ Pseudomonas sp. inducible cyclohexanone + NADH
-
?
cyclohexanol + NAD+ Pseudomonas sp. metabolism of cyclohexane cyclohexanone + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.8
-
oxidation of cyclohexanol at pH 7.8 Pseudomonas sp.
1.9
-
reduction of cyclohexanone at pH 7.8 Pseudomonas sp.
2.5
-
pH 10 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, stable for several days Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-cycloheptanediol + NAD+ relative oxidation activity at 25% the rate of cyclohexanol oxidation Pseudomonas sp. cycloheptane-1,2-dione + NADH
-
?
1-heptanol + NAD+ relative oxidation activity at 32% the rate of cyclohexanol oxidation Pseudomonas sp. heptanal + NADH
-
?
1-hexanol + NAD+ relative oxidation activity at 7% the rate of cyclohexanol oxidation Pseudomonas sp. hexanal + NADH
-
?
2-cyclohexanol + NAD+ relative oxidation activity at 142% the rate of cyclohexanol oxidation Pseudomonas sp. 2-cyclohexenone + NADH slow reduction r
2-hydroxycyclohexanone + NADH relative activity at 50% the rate of cyclohexanone reduction Pseudomonas sp. ? + NAD+
-
?
cycloheptanone + NADH relative activity at 10% the rate of cyclohexanone reduction Pseudomonas sp. cycloheptanol + NAD+
-
?
cyclohexan-1,2-diol + NAD+ relative oxidation activity at 15% the rate of cyclohexanol oxidation Pseudomonas sp. cyclohexane-1,2-dione + NADH slow reduction r
cyclohexane-1,3-diol + NAD+ relative oxidation activity at 14% the rate of cyclohexanol oxidation Pseudomonas sp. cyclohexane-1,3-dione + NADH slow reduction r
cyclohexane-1,4-diol + NAD+ relative oxidation activity at 36% the rate of cyclohexanol oxidation Pseudomonas sp. cyclohexane-1,4-dione + NADH relative reduction activity at 240% the rate of cyclohexanone reduction r
cyclohexanol + NAD+
-
Pseudomonas sp. cyclohexanone + NADH
-
r
cyclohexanol + NAD+ inducible Pseudomonas sp. cyclohexanone + NADH
-
?
cyclohexanol + NAD+ metabolism of cyclohexane Pseudomonas sp. cyclohexanone + NADH
-
?
cyclopentanol + NAD+
-
Pseudomonas sp. cyclopentanone + NADH relative reduction activity at 4% the rate of cyclohexanone reduction r
additional information 2-hydroxycyclohexanone, 1,3,5-trihydroxycyclohexane, menthol, ethanol, 1-propanol, 1-butanol and 1-pentanol are not oxidized with NAD+ Pseudomonas sp. additional information 2-cyclopentenone, 1,3-cyclohexanedione, 1,3-cyclopentanedione and 5-oxocapronate are not reduced with NADH ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
10.5
-
rapid inactivation above pH 10.5 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas sp.
NADP+ less than 5% activity than with NAD+ Pseudomonas sp.