Protein Variants | Comment | Organism |
---|---|---|
E396V | mutant enzyme has superior methanol conversion efficiency, with 79fold improvements compared to the wild-type | Lysinibacillus xylanilyticus |
E396V | mutant enzyme shows high activity, particularly at very low methanol concentrations | Lysinibacillus xylanilyticus |
K318N | mutant enzyme has superior methanol conversion efficiency, with 23fold improvements compared to the wild-type | Lysinibacillus xylanilyticus |
K318N | mutant enzyme shows high activity, particularly at very low methanol concentrations | Lysinibacillus xylanilyticus |
K46E | mutant enzyme has superior methanol conversion efficiency, with 3fold improvements compared to the wild-type | Lysinibacillus xylanilyticus |
K46E | mutant enzyme shows high activity, particularly at very low methanol concentrations | Lysinibacillus xylanilyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
methanol | pH 9.5, 55°C, mutant enzyme E396V | Lysinibacillus xylanilyticus | |
0.046 | - |
methanol | pH 9.5, 55°C, mutant enzyme K318N | Lysinibacillus xylanilyticus | |
0.233 | - |
methanol | pH 9.5, 55°C, mutant enzyme E396V/K318N | Lysinibacillus xylanilyticus | |
0.372 | - |
methanol | pH 9.5, 55°C, mutant enzyme K46E | Lysinibacillus xylanilyticus | |
1.311 | - |
methanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | Lysinibacillus xylanilyticus | - |
formaldehyde + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysinibacillus xylanilyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lysinibacillus xylanilyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | - |
Lysinibacillus xylanilyticus | formaldehyde + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lxmdh | - |
Lysinibacillus xylanilyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
methanol | pH 9.5, 55°C, mutant enzyme E396V | Lysinibacillus xylanilyticus | |
0.022 | - |
methanol | pH 9.5, 55°C, mutant enzyme E396V/K318N | Lysinibacillus xylanilyticus | |
0.027 | - |
methanol | pH 9.5, 55°C, mutant enzyme K318N | Lysinibacillus xylanilyticus | |
0.028 | - |
methanol | pH 9.5, 55°C, mutant enzyme K46E | Lysinibacillus xylanilyticus | |
0.0333 | - |
methanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Lysinibacillus xylanilyticus |
General Information | Comment | Organism |
---|---|---|
metabolism | methanol dehydrogenase, is a crucial enzyme for utilizing methane and methanol as carbon and energy sources in methylotrophy and synthetic methylotrophy | Lysinibacillus xylanilyticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
methanol | pH 9.5, 55°C, wild-type enzyme | Lysinibacillus xylanilyticus | |
0.076 | - |
methanol | pH 9.5, 55°C, mutant enzyme K46E | Lysinibacillus xylanilyticus | |
0.093 | - |
methanol | pH 9.5, 55°C, mutant enzyme E396V/K318N | Lysinibacillus xylanilyticus | |
0.58 | - |
methanol | pH 9.5, 55°C, mutant enzyme K318N | Lysinibacillus xylanilyticus | |
2 | - |
methanol | pH 9.5, 55°C, mutant enzyme E396V | Lysinibacillus xylanilyticus |