Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Activator protein | activation in presence of Mg2+, in vitro: 3 mol soluble activator protein per mol enzyme, in vivo estimated as 1 mol per 17.5 mol enzyme, MW 50000 with two subunits of MW 27000 each, in presence of activator protein and Mg2+ enzyme possesses a high affinity active site for alcohol and NAD+, up to 40fold increase of activity, primarily of Vmax, slight decrease of Km-value for methanol, enzyme/activator-interaction is dilution-sensitive, no stimulation of formaldehyde reduction | Bacillus methanolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | biphasic kinetics with all alcoholic substrates and NAD+ in the presence of activator protein | Bacillus methanolicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bacillus methanolicus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, 1-2 mol per mol subunit, atomic absorption spectroscopy, activation by activator protein depends on Mg2+, Zn2+, Mn2+, Ca2+ cannot replace Mg2+ | Bacillus methanolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | Bacillus methanolicus | involved in initial methanol oxidation | formaldehyde + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus methanolicus | - |
thermotolerant methylotrophic strain C1 | - |
Purification (Comment) | Organism |
---|---|
purification of activator protein | Bacillus methanolicus |
Storage Stability | Organism |
---|---|
4-20°C, complete dissociation after 48 h | Bacillus methanolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + NAD+ | no activity with 2-propanol, 2,3-butandiol, mannitol and glycerol | Bacillus methanolicus | formaldehyde + NADH + H+ | - |
? | |
methanol + NAD+ | involved in initial methanol oxidation | Bacillus methanolicus | formaldehyde + NADH + H+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | 59 | - |
Bacillus methanolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
t1/2: above 2 min | Bacillus methanolicus |
60 | - |
t1/2: 6 min | Bacillus methanolicus |
70 | - |
t1/2: 2 min | Bacillus methanolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
methanol oxidation | Bacillus methanolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required, specific for | Bacillus methanolicus |