Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Corynebacterium glutamicum |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion sitting-drop method at 20°C. Atomic resolution crystal structures of the enzyme in different functional states: with bound NAD+ (binary complex) and as ternary complexes with NADH plus either shikimate or quinate | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NAD+ | 30°C, pH 7.5 | Corynebacterium glutamicum | |
0.28 | - |
NAD+ | 30°C, pH 9.0-9.5, cosubstrate: quinate | Corynebacterium glutamicum | |
0.46 | - |
NAD+ | 30°C, pH 10.0-10.5, cosubstrate: shikimate | Corynebacterium glutamicum | |
0.87 | - |
NAD+ | 30°C, pH 7.5 | Corynebacterium glutamicum | |
1.6 | - |
L-quinate | 30°C, pH 7.5 | Corynebacterium glutamicum | |
2.4 | - |
L-quinate | 30°C, pH 9.0-9.5 | Corynebacterium glutamicum | |
10.2 | - |
shikimate | 30°C, pH 7.5 | Corynebacterium glutamicum | |
53.9 | - |
shikimate | 30°C, pH 10.0-10.5 | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-quinate + NAD+ | Corynebacterium glutamicum | the enzyme is involved in the catabolic quinate metabolism required for the degradation of lignin | 3-dehydroquinate + NADH + H+ | - |
r | |
L-quinate + NAD+ | Corynebacterium glutamicum ATCC 13032 | the enzyme is involved in the catabolic quinate metabolism required for the degradation of lignin | 3-dehydroquinate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q9X5C9 | - |
- |
Corynebacterium glutamicum ATCC 13032 | Q9X5C9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-quinate + NAD+ | the enzyme is involved in the catabolic quinate metabolism required for the degradation of lignin | Corynebacterium glutamicum | 3-dehydroquinate + NADH + H+ | - |
r | |
L-quinate + NAD+ | the enzyme also shows activity with shikimate. Clear substrate preference of the enzyme for quinate compared with shikimate both at the pH optimum and in a physiological pH range. The enzyme is strictly NAD(H) dependent | Corynebacterium glutamicum | 3-dehydroquinate + NADH + H+ | - |
r | |
L-quinate + NAD+ | the enzyme is involved in the catabolic quinate metabolism required for the degradation of lignin | Corynebacterium glutamicum ATCC 13032 | 3-dehydroquinate + NADH + H+ | - |
r | |
L-quinate + NAD+ | the enzyme also shows activity with shikimate. Clear substrate preference of the enzyme for quinate compared with shikimate both at the pH optimum and in a physiological pH range. The enzyme is strictly NAD(H) dependent | Corynebacterium glutamicum ATCC 13032 | 3-dehydroquinate + NADH + H+ | - |
r | |
shikimate + NAD+ | the enzyme also shows high activity with quinate. Clear substrate preference of the enzyme for quinate compared with shikimate both at the pH optimum and in a physiological pH range. The enzyme is strictly NAD(H) dependent | Corynebacterium glutamicum | 3-dehydroshikimate + NADH + H+ | - |
r | |
shikimate + NAD+ | the enzyme also shows high activity with quinate. Clear substrate preference of the enzyme for quinate compared with shikimate both at the pH optimum and in a physiological pH range. The enzyme is strictly NAD(H) dependent | Corynebacterium glutamicum ATCC 13032 | 3-dehydroshikimate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
cgl0424 | - |
Corynebacterium glutamicum |
CglQSDH | - |
Corynebacterium glutamicum |
quinate/shikimate dehydrogenase | the enzyme also shows activity with shikimate | Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Corynebacterium glutamicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30.1 | - |
shikimate | 30°C, pH 7.5 | Corynebacterium glutamicum | |
43.7 | - |
NAD+ | 30°C, pH 7.5 | Corynebacterium glutamicum | |
50.8 | - |
L-quinate | 30°C, pH 7.5 | Corynebacterium glutamicum | |
61.1 | - |
NAD+ | 30°C, pH 7.5 | Corynebacterium glutamicum | |
104.9 | - |
L-quinate | 30°C, pH 9.0-9.5 | Corynebacterium glutamicum | |
205.8 | - |
NAD+ | 30°C, pH 10.0-10.5, cosubstrate: shikimate | Corynebacterium glutamicum | |
214.1 | - |
shikimate | 30°C, pH 10.0-10.5 | Corynebacterium glutamicum | |
223.1 | - |
NAD+ | 30°C, pH 9.0-9.5, cosubstrate: quinate | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.5 | substrate: L-quinate | Corynebacterium glutamicum |
10 | 10.5 | substrate: shikimate | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strictly dependent on | Corynebacterium glutamicum | |
NADH | strictly dependent on | Corynebacterium glutamicum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
shikimate | 30°C, pH 7.5 | Corynebacterium glutamicum | |
4.2 | - |
shikimate | 30°C, pH 10.0-10.5 | Corynebacterium glutamicum | |
33.3 | - |
L-quinate | 30°C, pH 7.5 | Corynebacterium glutamicum | |
44.05 | - |
L-quinate | 30°C, pH 9.0-9.5 | Corynebacterium glutamicum | |
71.6 | - |
NAD+ | 30°C, pH 7.5 | Corynebacterium glutamicum | |
329.1 | - |
NAD+ | 30°C, pH 7.5 | Corynebacterium glutamicum | |
464.7 | - |
NAD+ | 30°C, pH 10.0-10.5, cosubstrate: shikimate | Corynebacterium glutamicum | |
826.6 | - |
NAD+ | 30°C, pH 9.0-9.5, cosubstrate: quinate | Corynebacterium glutamicum |