Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Neurospora crassa |
General Stability | Organism |
---|---|
high ionic strength, such as 0.1 M (NH4)2SO4, NaCl or phosphate buffer or the presence of 0.1 M quinate or shikimate protects against thermal inactivation | Neurospora crassa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | - |
Neurospora crassa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | 0.37 | quinate | - |
Neurospora crassa | |
1.18 | - |
shikimate | - |
Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
1 * 41000, SDS-PAGE in presence of urea or electrophoresis in presence of cetyltrimethyl ammonium bromide | Neurospora crassa |
42000 | - |
sucrose density gradient centrifugation | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinate + NAD+ | Neurospora crassa | first reaction in inducible quinic acid catabolic pathway | 5-dehydroquinate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
bifunctional enzyme: quinate (shikimate) dehydrogenase | - |
Purification (Comment) | Organism |
---|---|
bifunctional enzyme: quinate (shikimate) dehydrogenase | Neurospora crassa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Neurospora crassa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
86.87 | - |
- |
Neurospora crassa |
Storage Stability | Organism |
---|---|
-20°C, 0.1 M NaCl, half-life 40 days | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinate + NAD+ | - |
Neurospora crassa | 5-dehydroquinate + NADH + H+ | - |
? | |
quinate + NAD+ | first reaction in inducible quinic acid catabolic pathway | Neurospora crassa | 5-dehydroquinate + NADH + H+ | - |
? | |
shikimate + NAD+ | - |
Neurospora crassa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE in presence of urea or electrophoresis in presence of cetyltrimethyl ammonium bromide | Neurospora crassa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life of unprotected enzyme: 20 min, 180 min in presence of NaCl, completely stable in presence of quinate, shikimate or NADH | Neurospora crassa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
-20°C, highest stability | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for NAD+ | Neurospora crassa | |
NADH | specific for NAD+ | Neurospora crassa |