Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Wickerhamia fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, less than 10% residual activity | Wickerhamia fluorescens | |
Fe2+ | 1 mM, less than 10% residual activity | Wickerhamia fluorescens | |
Hg2+ | 1 mM, less than 10% residual activity | Wickerhamia fluorescens | |
WO42- | 1 mM, less than 10% residual activity | Wickerhamia fluorescens | |
Zn2+ | 1 mM, less than 10% residual activity | Wickerhamia fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | pH 6.5, 25°C | Wickerhamia fluorescens | |
0.1 | - |
NADH | pH 6.5, 25°C | Wickerhamia fluorescens | |
0.4 | - |
phenylpyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
0.64 | - |
hydroxyphenylpyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
3.5 | - |
Hydroxypyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
18.9 | - |
glyoxylate | pH 6.5, 25°C | Wickerhamia fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE, 2 * 40300, calculated | Wickerhamia fluorescens |
40300 | - |
2 * 40000, SDS-PAGE, 2 * 40300, calculated | Wickerhamia fluorescens |
75000 | - |
gel filtration | Wickerhamia fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Wickerhamia fluorescens | F1T2J9 | - |
- |
Wickerhamia fluorescens TK1 | F1T2J9 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
727 | - |
pH 6.5, 25°C | Wickerhamia fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + NADPH + H+ | - |
Wickerhamia fluorescens | D-(4-hydroxyphenyl)lactate + NADP+ | - |
? | |
4-hydroxyphenylpyruvate + NADPH + H+ | - |
Wickerhamia fluorescens TK1 | D-(4-hydroxyphenyl)lactate + NADP+ | - |
? | |
glyoxylate + NADPH + H+ | - |
Wickerhamia fluorescens | glycolate + NADP+ | - |
? | |
glyoxylate + NADPH + H+ | - |
Wickerhamia fluorescens TK1 | glycolate + NADP+ | - |
? | |
hydroxypyruvate + NADH + H+ | - |
Wickerhamia fluorescens | D-glycerate + NAD+ | - |
? | |
hydroxypyruvate + NADH + H+ | - |
Wickerhamia fluorescens TK1 | D-glycerate + NAD+ | - |
? | |
additional information | no substrates: pyruvate, oxaloacetate or benzoylformate | Wickerhamia fluorescens | ? | - |
? | |
additional information | no substrates: pyruvate, oxaloacetate or benzoylformate | Wickerhamia fluorescens TK1 | ? | - |
? | |
phenylpyruvate + NADPH + H+ | more than 99.9% D-isomer, L-isomer below limits of detection | Wickerhamia fluorescens | D-phenyllactate + NADP+ | - |
? | |
phenylpyruvate + NADPH + H+ | more than 99.9% D-isomer, L-isomer below limits of detection | Wickerhamia fluorescens TK1 | D-phenyllactate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE, 2 * 40300, calculated | Wickerhamia fluorescens |
Synonyms | Comment | Organism |
---|---|---|
Ppr | - |
Wickerhamia fluorescens |
pprA | - |
Wickerhamia fluorescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.1 | - |
Hydroxypyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
19 | - |
glyoxylate | pH 6.5, 25°C | Wickerhamia fluorescens | |
31 | - |
NADH | pH 6.5, 25°C | Wickerhamia fluorescens | |
73 | - |
hydroxyphenylpyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
121 | - |
NADPH | pH 6.5, 25°C | Wickerhamia fluorescens | |
150 | - |
phenylpyruvate | pH 6.5, 25°C | Wickerhamia fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Wickerhamia fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Wickerhamia fluorescens | |
NADH | NADPH is preferred over NADH | Wickerhamia fluorescens | |
NADP+ | - |
Wickerhamia fluorescens | |
NADPH | preferred over NADH | Wickerhamia fluorescens |
Organism | Comment | Expression |
---|---|---|
Wickerhamia fluorescens | presence of phenylalanine results in up to 40fold increase in transcripts | up |
General Information | Comment | Organism |
---|---|---|
physiological function | Wickerhamia fluorescens efficiently converts phenylalanine and phenylpyruvate to D-phenyllactate. These compounds up-regulate the transcription of enzyme gene pprA | Wickerhamia fluorescens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
glyoxylate | pH 6.5, 25°C | Wickerhamia fluorescens | |
2.6 | - |
Hydroxypyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
110 | - |
hydroxyphenylpyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
310 | - |
NADH | pH 6.5, 25°C | Wickerhamia fluorescens | |
380 | - |
phenylpyruvate | pH 6.5, 25°C | Wickerhamia fluorescens | |
12000 | - |
NADPH | pH 6.5, 25°C | Wickerhamia fluorescens |