Cloned (Comment) | Organism |
---|---|
TRII cDNA subcloned from pTR2EN induced in Escherichia coli BL21(DE3) | Datura stramonium |
Crystallization (Comment) | Organism |
---|---|
TrII is crystallized by hanging-drop vapor diffusion with 2-methyl-2,4-pentanediol as precipitating reagent, crystals grown by macroseeding technique, crystals belong to the P4.2.2.1.2 space group with cell dimensions of a = b = 62.8 A and c = 128.4 A | Datura stramonium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropinone + NADPH + H+ | Datura stramonium | - |
pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | Hyoscyamus niger | - |
pseudotropine + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Datura stramonium | - |
- |
- |
Hyoscyamus niger | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tropinone + NADPH + H+ | - |
Datura stramonium | pseudotropine + NADP+ | - |
? | |
tropinone + NADPH + H+ | - |
Hyoscyamus niger | pseudotropine + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Datura stramonium |
dimer | - |
Hyoscyamus niger |