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Literature summary for 1.1.1.236 extracted from

  • Portsteffen, A.; Draeger, B.; Nahrstedt, A.
    Two tropinone reducing enzymes from Datura stramonium transformed root cultures (1992), Phytochemistry, 31, 1135-1138.
No PubMed abstract available

General Stability

General Stability Organism
activity rapidly lost during purification Datura stramonium
activity rapidly lost during purification Atropa belladonna
activity rapidly lost during purification Hyoscyamus niger

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoic acid
-
Hyoscyamus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0061
-
NADPH
-
Hyoscyamus niger
0.0076
-
NADH
-
Hyoscyamus niger
0.017
-
NADPH
-
Datura stramonium
0.0211
-
NADPH
-
Hyoscyamus niger
0.023
-
NADPH
-
Datura stramonium
0.034
-
tropinone
-
Hyoscyamus niger
0.0351
-
tropinone
-
Hyoscyamus niger
0.22
-
tropinone
-
Datura stramonium
0.251
-
NADP+
-
Hyoscyamus niger
0.265
-
N-propylyl-4-piperidone
-
Hyoscyamus niger
0.534
-
4-ethylcyclohexanone
-
Hyoscyamus niger
0.77
-
N-Methyl-4-piperidone
-
Hyoscyamus niger
0.83
-
tropinone
-
Datura stramonium
2.03
-
4-methylcyclohexanone
-
Hyoscyamus niger
7.58
-
3-methylcyclohexanone
-
Hyoscyamus niger
21.6
-
4-Piperidone
-
Hyoscyamus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000 55000 TRII preparation is not yet pure and shows numerous bands Datura stramonium
29000
-
3 * 29000, SDS-PAGE Hyoscyamus niger
29000
-
4 * 29000, SDS-PAGE Hyoscyamus niger
69000
-
minor peak, gel filtration Hyoscyamus niger
103000
-
major peak, gel filtration Hyoscyamus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tropinone + NADPH + H+ Datura inoxia
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Datura stramonium
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Atropa belladonna
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Hyoscyamus muticus
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Hyoscyamus pusillus
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Hyoscyamus canariensis
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Hyoscyamus bohemicus
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Physochlaina orientalis
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Physalis philadelphica
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Physalis edulis
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Calystegia sepium
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Alkekengi officinarum
-
pseudotropine + NADP+
-
?
tropinone + NADPH + H+ Hyoscyamus niger reverse reaction very inefficient, about 1.3% of the forward activity pseudotropine + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Alkekengi officinarum
-
-
-
Atropa belladonna
-
-
-
Calystegia sepium
-
-
-
Datura inoxia
-
-
-
Datura stramonium
-
-
-
Hyoscyamus bohemicus
-
-
-
Hyoscyamus canariensis
-
-
-
Hyoscyamus muticus
-
-
-
Hyoscyamus niger
-
-
-
Hyoscyamus pusillus
-
-
-
no activity in Brassica campestris
-
-
-
no activity in Browallia americana
-
-
-
no activity in Nicotiana tabacum
-
tobacco
-
Physalis edulis
-
-
-
Physalis philadelphica
-
-
-
Physochlaina orientalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Datura stramonium
-
Hyoscyamus niger

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Atropa belladonna
-
leaf
-
Atropa belladonna
-
root
-
Datura inoxia
-
root
-
Atropa belladonna
-
root
-
Hyoscyamus niger
-
root
-
Physalis philadelphica
-
root
-
Physalis edulis
-
root
-
Calystegia sepium
-
root
-
Alkekengi officinarum
-
root hairy roots Datura stramonium
-
stem
-
Datura stramonium
-
stem
-
Atropa belladonna
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.79
-
-
Datura stramonium

Storage Stability

Storage Stability Organism
-20°C, stable for at least 3 months Datura stramonium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyl-cyclohexanone + NADPH
-
Hyoscyamus niger 2-methylcyclohexanol + NADP+
-
?
3-methyl-cyclohexanone + NADPH
-
Hyoscyamus niger 3-methylcyclohexanol + NADP+
-
?
4-ethyl-cyclohexanone + NADPH
-
Datura stramonium 4-ethylcyclohexanol + NADP+
-
?
4-ethyl-cyclohexanone + NADPH
-
Hyoscyamus niger 4-ethylcyclohexanol + NADP+
-
?
4-methyl-cyclohexanone + NADPH
-
Datura stramonium 4-methylcyclohexanol + NADP+
-
?
4-methyl-cyclohexanone + NADPH
-
Hyoscyamus niger 4-methylcyclohexanol + NADP+
-
?
4-piperidone + NADPH
-
Hyoscyamus niger piperidine-4-ol + NADP+
-
?
6-hydroxytropinone + NADPH
-
Datura stramonium ? + NADP+
-
?
6-hydroxytropinone + NADPH
-
Hyoscyamus niger ? + NADP+
-
?
N-methyl-4-piperidone + NADPH
-
Datura stramonium N-methylpiperidin-4-ol + NADP+
-
?
N-methyl-4-piperidone + NADPH
-
Hyoscyamus niger N-methylpiperidin-4-ol + NADP+
-
?
N-propyl-4-piperidone + NADPH
-
Datura stramonium N-propylpiperidin-4-ol + NADP+
-
?
N-propyl-4-piperidone + NADPH
-
Hyoscyamus niger N-propylpiperidin-4-ol + NADP+
-
?
tetrahydrothiopyran-4-one + NADPH
-
Datura stramonium tetrahydrothiopyran-4-ol + NADP+
-
?
tetrahydrothiopyran-4-one + NADPH
-
Hyoscyamus niger tetrahydrothiopyran-4-ol + NADP+
-
?
tropinone + NADPH + H+
-
Datura inoxia pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Datura stramonium pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Atropa belladonna pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Hyoscyamus niger pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Hyoscyamus muticus pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Hyoscyamus pusillus pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Hyoscyamus canariensis pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Hyoscyamus bohemicus pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Physochlaina orientalis pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Physalis philadelphica pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Physalis edulis pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Calystegia sepium pseudotropine + NADP+
-
?
tropinone + NADPH + H+
-
Alkekengi officinarum pseudotropine + NADP+
-
?
tropinone + NADPH + H+ reverse reaction very inefficient, about 1.3% of the forward activity Hyoscyamus niger pseudotropine + NADP+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 29000, SDS-PAGE Hyoscyamus niger
trimer
-
Datura stramonium
trimer
-
Atropa belladonna
trimer 3 * 29000, SDS-PAGE Hyoscyamus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3 6.5
-
Hyoscyamus niger
5.8
-
forward reaction Hyoscyamus niger

pH Range

pH Minimum pH Maximum Comment Organism
4 5 reverse oxidative activities Hyoscyamus niger
4.5 8.5 tolerant to pH changes over a wide range Datura stramonium

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.8 8.5 more acidic buffers inactivate the enzyme highly, 8% of the maximum activity at pH 4.5, more alkaline buffers inhibit the activity less strongly, 61% of the maximum activity at pH 8.5 Hyoscyamus niger

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Hyoscyamus niger
NADPH activity exclusively with NADPH, not active with NADH Datura stramonium