KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
8-Ketocoformycin | - |
Streptomyces antibioticus | |
0.25 | - |
8-Ketodeoxycoformycin | - |
Streptomyces antibioticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-ketocoformycin + NADPH | Streptomyces antibioticus | production of the antibiotic coformycin | coformycin + NADP+ | - |
r | |
8-ketodeoxycoformycin + NADPH | Streptomyces antibioticus | production of the antibiotic 2'-deoxycoformycin | 2'-deoxycoformycin + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces antibioticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Streptomyces antibioticus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
coformycin + NADP+ = 8-oxocoformycin + NADPH + H+ | possible mechanism | Streptomyces antibioticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-ketocoformycin + NADPH | stereospecifically reduces the 8-keto group of the ketonucleoside substrate to a hydroxyl group with the R configuration at C-8 | Streptomyces antibioticus | coformycin + NADP+ | - |
r | |
8-ketocoformycin + NADPH | production of the antibiotic coformycin | Streptomyces antibioticus | coformycin + NADP+ | - |
r | |
8-ketodeoxycoformycin + NADPH | stereospecifically reduces the 8-keto group of the ketonucleoside substrate to a hydroxyl group with the R configuration at C-8 | Streptomyces antibioticus | 2'-deoxycoformycin + NADP+ | - |
r | |
8-ketodeoxycoformycin + NADPH | production of the antibiotic 2'-deoxycoformycin | Streptomyces antibioticus | 2'-deoxycoformycin + NADP+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Streptomyces antibioticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
no activity | Streptomyces antibioticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Streptomyces antibioticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADPH is 25-fold higher active than NADH | Streptomyces antibioticus | |
NADPH | B-specific with respect to NADPH, 25-fold higher activity than with NADH | Streptomyces antibioticus |