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Literature summary for 1.1.1.227 extracted from

  • Hofer, M.; Diener, J.; Begander, B.; Kourist, R.; Sieber, V.
    Engineering of a borneol dehydrogenase from P. putida for the enzymatic resolution of camphor (2021), Appl. Microbiol. Biotechnol., 105, 3159-3167 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.227

Protein Variants

Protein Variants Comment Organism
Y188A mutant catalyzes stereoselective reduction of racemic camphor with 23% conversion and 99% enantiomeric excess Pseudomonas putida
Y188T mutant catalyzes stereoselective reduction of racemic camphor with 22% conversion and 99% enantiomeric excess Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 NAD+ wild-type, pH 7.5, 30°C Pseudomonas putida
0.11
-
 (-)-borneol wild-type, pH 7.5, 30°C Pseudomonas putida
0.34
-
 (-)-borneol mutant Y188A, pH 7.5, 30°C Pseudomonas putida
0.73
-
 (-)-borneol mutant Y188T, pH 7.5, 30°C Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida ATCC 17453
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-borneol + NAD+
-
 Pseudomonas putida (-)-camphor + NADH + H+
-
 r
(-)-camphor + NADH + H+
-
 Pseudomonas putida (-)-borneol + NAD+
-
 r
additional information additional substrates are (+)-borneol, (+)-camphor and (+)-isoborneol, reaction of EC 1.1.1.198. No substrates: isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, 2-butanol, L-carveol, and DL-menthol Pseudomonas putida ?
-
-
additional information additional substrates are (+)-borneol, (+)-camphor and (+)-isoborneol, reaction of EC 1.1.1.198. No substrates: isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, 2-butanol, L-carveol, and DL-menthol Pseudomonas putida ATCC 17453 ?
-
-

Subunits

Subunits Comment Organism
? x * 27500, calculated from sequence, x * 31000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
BDH cf. EC 1.1.1.198 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 (-)-borneol mutant Y188A, pH 7.5, 30°C Pseudomonas putida
0.13
-
 (-)-borneol mutant Y188T, pH 7.5, 30°C Pseudomonas putida
0.97
-
 (-)-borneol wild-type, pH 7.5, 30°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 reduction of camphor Pseudomonas putida
7.5
-
 oxidation of borneol Pseudomonas putida

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas putida calculated from sequence
-
 5.4