Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | slight inhibition | Corynebacterium cyclohexanicum | |
iodoacetic acid | slight inhibition | Corynebacterium cyclohexanicum | |
N-bromosuccinimide | strong inhibition | Corynebacterium cyclohexanicum | |
p-hydroxymercuribenzoate | slight inhibition | Corynebacterium cyclohexanicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
NAD+ | - |
Corynebacterium cyclohexanicum | |
0.28 | - |
NADH | - |
Corynebacterium cyclohexanicum | |
0.5 | - |
4-Oxocyclohexanecarboxylate | - |
Corynebacterium cyclohexanicum | |
0.51 | - |
trans-4-hydroxycyclohexanecarboxylate | - |
Corynebacterium cyclohexanicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27600 | - |
SDS-PAGE | Corynebacterium cyclohexanicum |
53600 | - |
gel filtration | Corynebacterium cyclohexanicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-4-hydroxycyclohexanecarboxylate + NAD+ | Corynebacterium cyclohexanicum | - |
4-oxocyclohexanecarboxylate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium cyclohexanicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium cyclohexanicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.48 | - |
crude extract, NADH oxidation | Corynebacterium cyclohexanicum |
40 | - |
purified enzyme, NAD reduction | Corynebacterium cyclohexanicum |
104.1 | - |
purified enzyme, NADH oxidation | Corynebacterium cyclohexanicum |
Storage Stability | Organism |
---|---|
-80°C | Corynebacterium cyclohexanicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trans-4-hydroxycyclohexanecarboxylate + NAD+ | - |
Corynebacterium cyclohexanicum | 4-oxocyclohexanecarboxylate + NADH | - |
r | |
trans-4-hydroxycyclohexanecarboxylate + NAD+ | higher specific activities assayed for reverse reaction | Corynebacterium cyclohexanicum | 4-oxocyclohexanecarboxylate + NADH | - |
r | |
trans-4-hydroxycyclohexanecarboxylate + NAD+ | highly specific for trans isomer | Corynebacterium cyclohexanicum | 4-oxocyclohexanecarboxylate + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 27600, gel filtration, SDS-PAGE | Corynebacterium cyclohexanicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7.2 | NADH oxidation, depending on the buffer system | Corynebacterium cyclohexanicum |
8 | - |
NAD reduction | Corynebacterium cyclohexanicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Corynebacterium cyclohexanicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | very specific, no activity with NADP+ | Corynebacterium cyclohexanicum | |
NADH | very specific, no activity with NADPH | Corynebacterium cyclohexanicum |