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Literature summary for 1.1.1.226 extracted from

  • Obata, H.; Uebayasi, M.; Kaneda, T.
    Purification and properties of 4-hydroxycyclohexanecarboxylate dehydrogenase from Corynebacterium cyclohexanicum (1988), Eur. J. Biochem., 174, 451-458.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide slight inhibition Corynebacterium cyclohexanicum
iodoacetic acid slight inhibition Corynebacterium cyclohexanicum
N-bromosuccinimide strong inhibition Corynebacterium cyclohexanicum
p-hydroxymercuribenzoate slight inhibition Corynebacterium cyclohexanicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
NAD+
-
Corynebacterium cyclohexanicum
0.28
-
NADH
-
Corynebacterium cyclohexanicum
0.5
-
4-Oxocyclohexanecarboxylate
-
Corynebacterium cyclohexanicum
0.51
-
trans-4-hydroxycyclohexanecarboxylate
-
Corynebacterium cyclohexanicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27600
-
SDS-PAGE Corynebacterium cyclohexanicum
53600
-
gel filtration Corynebacterium cyclohexanicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trans-4-hydroxycyclohexanecarboxylate + NAD+ Corynebacterium cyclohexanicum
-
4-oxocyclohexanecarboxylate + NADH
-
r

Organism

Organism UniProt Comment Textmining
Corynebacterium cyclohexanicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Corynebacterium cyclohexanicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.48
-
crude extract, NADH oxidation Corynebacterium cyclohexanicum
40
-
purified enzyme, NAD reduction Corynebacterium cyclohexanicum
104.1
-
purified enzyme, NADH oxidation Corynebacterium cyclohexanicum

Storage Stability

Storage Stability Organism
-80°C Corynebacterium cyclohexanicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-4-hydroxycyclohexanecarboxylate + NAD+
-
Corynebacterium cyclohexanicum 4-oxocyclohexanecarboxylate + NADH
-
r
trans-4-hydroxycyclohexanecarboxylate + NAD+ higher specific activities assayed for reverse reaction Corynebacterium cyclohexanicum 4-oxocyclohexanecarboxylate + NADH
-
r
trans-4-hydroxycyclohexanecarboxylate + NAD+ highly specific for trans isomer Corynebacterium cyclohexanicum 4-oxocyclohexanecarboxylate + NADH
-
r

Subunits

Subunits Comment Organism
dimer 2 * 27600, gel filtration, SDS-PAGE Corynebacterium cyclohexanicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8 7.2 NADH oxidation, depending on the buffer system Corynebacterium cyclohexanicum
8
-
NAD reduction Corynebacterium cyclohexanicum

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Corynebacterium cyclohexanicum

Cofactor

Cofactor Comment Organism Structure
NAD+ very specific, no activity with NADP+ Corynebacterium cyclohexanicum
NADH very specific, no activity with NADPH Corynebacterium cyclohexanicum