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Literature summary for 1.1.1.224 extracted from

  • Song, G.Q.; Sink, K.C.; Ma, Y.; Herlache, T.; Hancock, J.F.; Loescher, W.H.
    A novel mannose-based selection system for plant transformation using celery mannose-6-phosphate reductase gene (2010), Plant Cell Rep., 29, 163-172.
    View publication on PubMed

Application

Application Comment Organism
agriculture transformation of mannose-6-phosphate reductase gene into Arabidopsis and tobacco using Agrobacterium tumefaciens-mediated transformation. Mannose-6-phosphate reductase can act as a selectable marker gene in either a positive or a negative selection mode depending on the plant species. On medium containing 2 g/l mannose, transgenic seeds germinate, whereas wild type seeds did not. Mannose at 30 g/l blanches leaf explants from all 29 transgenic tobacco events with mannose-6-phosphate reductase. In contrast, 30 g/l mannose does not inhibit shoot regeneration from leaf explants of wild-type or transgenic plants with either an antisense mannose-6-phosphate reductase or a plasmid control. Mannose at 30 g/l inhibits seed germination of transgenic tobacco seeds with mannose-6-phosphate reductase but not that of wild-type or transgenic tobacco with either the antisense mannose-6-phosphate reductase or the plasmid control Apium graveolens

Organism

Organism UniProt Comment Textmining
Apium graveolens
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