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Literature summary for 1.1.1.224 extracted from

  • Delavault, P.; Simier, P.; Thoiron, S.; Veronesi, C.; Fer, A.; Thalouarn, P.
    Isolation of mannose 6-phosphate reductase cDNA, changes in enzyme activity and mannitol content in broomrape (Orobanche ramosa) parasitic on tomato roots (2002), Physiol. Plant., 115, 48-55.
    View publication on PubMed

Application

Application Comment Organism
agriculture enzyme is a target for herbizide treatment Phelipanche ramosa

Cloned(Commentary)

Cloned (Comment) Organism
-
Phelipanche ramosa
single gene, constitutive, DNA sequence determination and analysis, expression in Escherichia coli Phelipanche ramosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
theoretical molecular weight, calculated from cDNA sequence Phelipanche ramosa
37000
-
1 * 37000, SDS-PAGE of recombinant enzyme Phelipanche ramosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mannitol 1-phosphate + NADP+ Phelipanche ramosa key enzyme in mannitol production in the parasite D-mannose 6-phosphate + NADPH + H+
-
?
D-mannose 6-phosphate + NADPH Phelipanche ramosa key enzyme in mannitol production in higher plants ?
-
?

Organism

Organism UniProt Comment Textmining
Phelipanche ramosa
-
broomrape
-
Phelipanche ramosa
-
parasitic broomrape on tomato roots
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant enzyme Phelipanche ramosa

Source Tissue

Source Tissue Comment Organism Textmining
additional information overview over different developmental stages Phelipanche ramosa
-
additional information constitutive gene expression during different developmental stages, e.g. germination and procaulome growth, of the parasite attached to tomato roots, high activity during tubercle enlargement and crown root formation Phelipanche ramosa
-
root
-
Phelipanche ramosa
-
seed
-
Phelipanche ramosa
-
shoot
-
Phelipanche ramosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
measurement of activity during plant development Phelipanche ramosa
0.00822
-
crude extract from expression in Escherichia coli Phelipanche ramosa
0.12
-
in tubercle Phelipanche ramosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol 1-phosphate + NADP+
-
Phelipanche ramosa D-mannose 6-phosphate + NADPH + H+
-
?
D-mannitol 1-phosphate + NADP+ key enzyme in mannitol production in the parasite Phelipanche ramosa D-mannose 6-phosphate + NADPH + H+
-
?
D-mannose 6-phosphate + NADPH key enzyme in mannitol production in higher plants Phelipanche ramosa ?
-
?
D-mannose 6-phosphate + NADPH
-
Phelipanche ramosa D-mannitol 1-phosphate + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE of recombinant enzyme Phelipanche ramosa

Synonyms

Synonyms Comment Organism
M6PR
-
Phelipanche ramosa
mannose 6-phosphate reductase
-
Phelipanche ramosa

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Phelipanche ramosa
NADPH
-
Phelipanche ramosa