Application | Comment | Organism |
---|---|---|
agriculture | enzyme is a target for herbizide treatment | Phelipanche ramosa |
Cloned (Comment) | Organism |
---|---|
- |
Phelipanche ramosa |
single gene, constitutive, DNA sequence determination and analysis, expression in Escherichia coli | Phelipanche ramosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
theoretical molecular weight, calculated from cDNA sequence | Phelipanche ramosa |
37000 | - |
1 * 37000, SDS-PAGE of recombinant enzyme | Phelipanche ramosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NADP+ | Phelipanche ramosa | key enzyme in mannitol production in the parasite | D-mannose 6-phosphate + NADPH + H+ | - |
? | |
D-mannose 6-phosphate + NADPH | Phelipanche ramosa | key enzyme in mannitol production in higher plants | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phelipanche ramosa | - |
broomrape | - |
Phelipanche ramosa | - |
parasitic broomrape on tomato roots | - |
Purification (Comment) | Organism |
---|---|
purification of recombinant enzyme | Phelipanche ramosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | overview over different developmental stages | Phelipanche ramosa | - |
additional information | constitutive gene expression during different developmental stages, e.g. germination and procaulome growth, of the parasite attached to tomato roots, high activity during tubercle enlargement and crown root formation | Phelipanche ramosa | - |
root | - |
Phelipanche ramosa | - |
seed | - |
Phelipanche ramosa | - |
shoot | - |
Phelipanche ramosa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
measurement of activity during plant development | Phelipanche ramosa |
0.00822 | - |
crude extract from expression in Escherichia coli | Phelipanche ramosa |
0.12 | - |
in tubercle | Phelipanche ramosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol 1-phosphate + NADP+ | - |
Phelipanche ramosa | D-mannose 6-phosphate + NADPH + H+ | - |
? | |
D-mannitol 1-phosphate + NADP+ | key enzyme in mannitol production in the parasite | Phelipanche ramosa | D-mannose 6-phosphate + NADPH + H+ | - |
? | |
D-mannose 6-phosphate + NADPH | key enzyme in mannitol production in higher plants | Phelipanche ramosa | ? | - |
? | |
D-mannose 6-phosphate + NADPH | - |
Phelipanche ramosa | D-mannitol 1-phosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, SDS-PAGE of recombinant enzyme | Phelipanche ramosa |
Synonyms | Comment | Organism |
---|---|---|
M6PR | - |
Phelipanche ramosa |
mannose 6-phosphate reductase | - |
Phelipanche ramosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Phelipanche ramosa | |
NADPH | - |
Phelipanche ramosa |