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Literature summary for 1.1.1.224 extracted from

  • Loescher, W.H.; Tyson, R.H.; Everard, J.D.; Redgewell, J.; Bieleski, R.L.
    Mannitol synthesis in higher plants. Evidence for the role and characterization of a NADPH-dependent mannose 6-phosphate reductase (1992), Plant Physiol., 98, 1396-1402.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.224

General Stability

General Stability Organism
degassed buffers stabilize Apium graveolens
DTT stabilizes Apium graveolens

Inhibitors

Inhibitors Comment Organism Structure
NaCl 47% reduction by 100 mM Apium graveolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15.8
-
 D-mannose 6-phosphate
-
 Apium graveolens
45
-
 D-mannitol 1-phosphate
-
 Apium graveolens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Apium graveolens 5737
-
cytoplasm
-
 Ligustrum vulgare 5737
-
protoplast
-
 Apium graveolens
-
-
protoplast
-
 Ligustrum vulgare
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Apium graveolens
53000
-
 gel filtration with Superose 12 Apium graveolens
58000
-
 gel filtration with Sephacryl S-200 Apium graveolens

Organism

Organism UniProt Comment Textmining
Apium graveolens
-
 celery
-
Apium graveolens
-
 cv. Giant Pascal
-
Ligustrum vulgare
-
 privet
-

Oxidation Stability

Oxidation Stability Organism
DTT stabilizes Apium graveolens

Purification (Commentary)

Purification (Comment) Organism
-
 Apium graveolens

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Apium graveolens
-
shoot
-
 Ligustrum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.053
-
-
 Apium graveolens
3.76
-
 purified from leaves Apium graveolens

Storage Stability

Storage Stability Organism
-21°C, 50% activity retained after 1 month Apium graveolens
4°C, lyophilized active fractions stable for several months Apium graveolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannose 6-phosphate + NADPH
-
 Apium graveolens D-mannitol 1-phosphate + NADP+
-
 r
D-mannose 6-phosphate + NADPH no activity with D-fructose 6-phosphate, D-glucose 6-phosphate, D-fructose 1-phosphate, D-mannose 1-phosphate, D-mannose Ligustrum vulgare D-mannitol 1-phosphate + NADP+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Apium graveolens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 D-mannose 6-phosphate Apium graveolens
8.5
-
 D-mannitol 1-phosphate Apium graveolens

pH Range

pH Minimum pH Maximum Comment Organism
6 9 inactivated at pH below 6 Apium graveolens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9
-
 Apium graveolens

Cofactor

Cofactor Comment Organism Structure
NADP+ NAD+ partially (8% activity) substitutes for NADP+ in oxidizing mannitol 1-phosphate Apium graveolens
NADP+ NAD+ partially (8% activity) substitutes for NADP+ in oxidizing mannitol 1-phosphate Ligustrum vulgare
NADPH NADH cannot replace NADPH in reducing mannose 6-phosphate Apium graveolens
NADPH NADH cannot replace NADPH in reducing mannose 6-phosphate Ligustrum vulgare