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Literature summary for 1.1.1.22 extracted from
- Biochemical characterization and functional analysis of Udp-glucose dehydrogenase, in the synthesis of biopolymer Ss from Sphingomonas sanxanigenens NX02 (2015), Appl. Biochem. Microbiol., 51, 27-33.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ugdG, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH6alpha | Sphingomonas sanxanigenens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | overexpression of the ugdG gene in Sphingomonas sanxanigenens results in increased sphingan Ss production and higher fermentation broth viscosity. The weightaverage molecular weight of polymer Ss from the recombinant strain is higher and the viscosity is higher than those from the wild-type strain at a shear rate of 1 rev/min | Sphingomonas sanxanigenens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Sphingomonas sanxanigenens | |
| 0.38 | - |
NAD+ | pH 8.7, 37°C, recombinant enzyme | Sphingomonas sanxanigenens |  |
| 0.47 | - |
UDP-alpha-D-glucose | pH 8.7, 37°C, recombinant enzyme | Sphingomonas sanxanigenens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48200 | - |
- |
Sphingomonas sanxanigenens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + 2 NAD+ + H2O | Sphingomonas sanxanigenens | - |
UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | UDP-alpha-D-glucuronate is a precursor in the synthesis of many exopolysaccharides | ? | |
| UDP-alpha-D-glucose + 2 NAD+ + H2O | Sphingomonas sanxanigenens NX02 | - |
UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | UDP-alpha-D-glucuronate is a precursor in the synthesis of many exopolysaccharides | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingomonas sanxanigenens | W0A856 | - |
- |
| Sphingomonas sanxanigenens NX02 | W0A856 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Sphingomonas sanxanigenens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + 2 NAD+ + H2O | - |
Sphingomonas sanxanigenens | UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | - |
? | |
| UDP-alpha-D-glucose + 2 NAD+ + H2O | - |
Sphingomonas sanxanigenens | UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | UDP-alpha-D-glucuronate is a precursor in the synthesis of many exopolysaccharides | ? | |
| UDP-alpha-D-glucose + 2 NAD+ + H2O | - |
Sphingomonas sanxanigenens NX02 | UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | - |
? | |
| UDP-alpha-D-glucose + 2 NAD+ + H2O | - |
Sphingomonas sanxanigenens NX02 | UDP-alpha-D-glucuronate + 2 NADH + 2 H+ | UDP-alpha-D-glucuronate is a precursor in the synthesis of many exopolysaccharides | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48200, about, sequence calculation | Sphingomonas sanxanigenens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucose dehydrogenase | - |
Sphingomonas sanxanigenens |
| UgdG | - |
Sphingomonas sanxanigenens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
recombinant enzyme | Sphingomonas sanxanigenens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 50 | purified recombinant His-tagged enzyme, activity range, profile overview | Sphingomonas sanxanigenens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 50 | the purified recombinant His-tagged enzyme is stable from 15°C to 30°C retaining more than 60% of its residual activity, while it retains only 28% activity at 50°C | Sphingomonas sanxanigenens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50.2 | - |
NAD+ | pH 8.7, 37°C, recombinant enzyme | Sphingomonas sanxanigenens | |
| 57.9 | - |
UDP-alpha-D-glucose | pH 8.7, 37°C, recombinant enzyme | Sphingomonas sanxanigenens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant enzyme | Sphingomonas sanxanigenens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | half-maximal activity at about pH 7.0 and pH 9.5, maximal activity at pH 8.0 | Sphingomonas sanxanigenens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Sphingomonas sanxanigenens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the the UGDH family of proteins | Sphingomonas sanxanigenens |
| additional information | the active site of UgdG bound to UDP-Glc and coenzyme NADH contains 6 highly conserved residues: Thr122, Glu151, Lys207, Asn211, Cys263 and Asp267. Residue Cys263 is a clear candidate for the catalytic nucleophile of the reaction. Tyr10 plays a catalytic role in the final hydrolysis of UDP-Glc | Sphingomonas sanxanigenens |
| physiological function | the enzyme is involved in the biosnthesis of the Ss sphingan biopolymer | Sphingomonas sanxanigenens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.05 | - |
UDP-alpha-D-glucose | pH 8.7, 37°C, recombinant enzyme | Sphingomonas sanxanigenens | |
| 2.2 | - |
NAD+ | pH 8.7, 37°C, recombinant enzyme | Sphingomonas sanxanigenens | |
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