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Literature summary for 1.1.1.22 extracted from

  • Rocha, J.; Popescu, A.O.; Sa-Correia, I.; Fialho, A.M.; Frazao, C.
    Cloning, expression, purification, crystallization and preliminary crystallographic studies of BceC, a UDP-glucose dehydrogenase from Burkholderia cepacia IST408 (2010), Acta Crystallogr. Sect. F, 66, 269-271.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene bceC, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Burkholderia cepacia

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His6-tagged BceC, 0.001 ml of protein solution containing 5 mg/ml protein in 25 mM Tris-HCl, pH 8.3, 50 mM NaCl, 2.5 mM DTT, 0.25 mM UDP-GlcA, and 0.5 mM NAD+, is mixed with 0.001 ml of precipitant solution containing 200 mM ammonium sulfate, 100 mM sodium acetate, pH 4.5, 11% w/v PEG 4000, and 50 mM NaF, method optimiization, X-ray diffraction structure determination and analysis at 2.09 A resolution, molecular replacement Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52600
-
x * 52600, recombinant His6-tagged BceC, SDS-PAGE Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + 2 NAD+ + H2O Burkholderia cepacia
-
UDP-glucuronic acid + 2 NADH + 2 H+
-
?
UDP-glucose + 2 NAD+ + H2O Burkholderia cepacia IST408
-
UDP-glucuronic acid + 2 NADH + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
gene bceC
-
Burkholderia cepacia IST408
-
gene bceC
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged BceC from Escherichia coli by affinity chromatography Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + 2 NAD+ + H2O
-
Burkholderia cepacia UDP-glucuronic acid + 2 NADH + 2 H+
-
?
UDP-glucose + 2 NAD+ + H2O
-
Burkholderia cepacia IST408 UDP-glucuronic acid + 2 NADH + 2 H+
-
?

Subunits

Subunits Comment Organism
? x * 52600, recombinant His6-tagged BceC, SDS-PAGE Burkholderia cepacia

Synonyms

Synonyms Comment Organism
BceC
-
Burkholderia cepacia
UDP-glucose dehydrogenase
-
Burkholderia cepacia
uridine-5'-diphosphoglucose dehydrogenase
-
Burkholderia cepacia

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Burkholderia cepacia