Application | Comment | Organism |
---|---|---|
medicine | possibility that gallic acid and quercetin may modulate human breast cancer cell proliferation by inhibiting UGDH | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
gallic acid | is a non-competitive inhibitor with respect to UDP-glucose and NAD+. It decreases specific activities of UGDH, but does not affect UGDH protein expression, thus UGDH activity is inhibited by polyphenols at the post-translational level. Gallic acid exerts strong antiproliferative activity in breast cancer cells. Heat inactivation of UGDH is accelerated to a greater degree by quercetin than by gallic acid. In the presence of gallic acid, the activity remaining after 30 min is 55% that of control | Homo sapiens | |
quercetin | shows a competitive inhibition and a mixed-type inhibition with respect to UDP-glucose and NAD+, decreases specific activities of UGDH, but does not affect UGDH protein expression, thus UGDH activity is inhibited by polyphenols at the post-translational level. Quercetin exerts strong antiproliferative activity in breast cancer cells. Heat inactivation of UGDH is accelerated to a greater degree by quercetin than by gallic acid. In the presence of quercetin, the activity remaining after 30 min is 20% that of control | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
UDP-glucose | - |
Homo sapiens | |
0.13 | - |
NAD+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MCF-7 cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00051 | - |
lysates of cells treated with 0.3 mM gallic acid | Homo sapiens |
0.00061 | - |
lysates of cells treated with 0.3 mM quercetin | Homo sapiens |
0.0015 | - |
control lysates | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 2 NAD+ + H2O | - |
Homo sapiens | UDP-glucuronate + 2 NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose dehydrogenase | - |
Homo sapiens |
UGDH | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
quercetin | with respect to NAD+ | Homo sapiens | |
0.083 | - |
quercetin | with respect to UDP-glucose | Homo sapiens | |
0.744 | - |
gallic acid | with respect to UDP-glucose | Homo sapiens | |
1.804 | - |
gallic acid | with respect to NAD+ | Homo sapiens |