Literature summary for 1.1.1.22 extracted from

Huh, J.W.; Yang, S.J.; Hwang, E.Y.; Choi, M.M.; Lee, H.J.; Kim, E.A.; Choi, S.Y.; Choi, J.; Hong, H.N.; Cho, S.W.
Alteration of the quaternary structure of human UDP-glucose dehydrogenase by a double mutation (2007), J. Biochem. Mol. Biol., 40, 690-696.

Search for more literature for 1.1.1.22

Protein Variants

Protein Variants	Comment	Organism
A222Q/S233G	mutation does not affect expression, stability, and secondary structure. Mutant protein is a dimer and catalytic active, with increased Km values for substrates	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.017	-	UDP	wild-type, pH 8.7, 22°C	Homo sapiens
0.017	-	UDP	wild type enzyme, at pH 8.7 and 25°C	Homo sapiens
0.133	-	NAD+	wild-type, pH 8.7, 22°C	Homo sapiens
0.401	-	NAD+	mutant A222Q/S233G, pH 8.7, 22°C	Homo sapiens
0.98	-	UDP	mutant A222Q/S233G, pH 8.7, 22°C	Homo sapiens
0.98	-	UDP	mutant enzyme A222Q/S233G, at pH 8.7 and 25°C	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
57000	-	gel filtration, minor part of wild-type	Homo sapiens
110000	-	gel filtration, minor part of wild-type, mutant A222Q/S233G	Homo sapiens
340000	-	gel filtration, wild-type	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-D-glucose + 2 NAD+ + H2O	-	Homo sapiens	UDP-alpha-D-glucuronate + 2 NADH + 2 H+	-	r
UDP-glucose + 2 NAD+ + H2O	-	Homo sapiens	UDP-glucuronate + 2 NADH + 2 H+	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 57000, mutant A222Q/S233G and part of wild-type, SDS-PAGE	Homo sapiens
hexamer	6* 57000, wild-type, plus some dimer and monomer, SDS-PAGE	Homo sapiens
monomer	1 * 57000, SDS-PAGE, minor part of wild-type, major part is hexamer	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
97	-	UDP	mutant A222Q/S233G, pH 8.7, 22°C	Homo sapiens
105	-	UDP	wild-type, pH 8.7, 22°C	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Homo sapiens
NADH	-	Homo sapiens

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Release 2023.1 (January 2023)