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Literature summary for 1.1.1.219 extracted from

  • Li, H.; Qiu, J.; Chen, F.; Lv, X.; Fu, C.; Zhao, D.; Hua, X.; Zhao, Q.
    Molecular characterization and expression analysis of dihydroflavonol 4-reductase (DFR) gene in Saussurea medusa (2012), Mol. Biol. Rep., 39, 2991-2999.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Saussurea medusa
gene SmDFR, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis, functional expression in Saccharomyces cerevisiae strain INV Sc1 Saussurea medusa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38600
-
x * 38600, SDS-PAGE Saussurea medusa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saussurea medusa the enzyme catalyzes the reduction of dihydroflavonols to leucoanthocyanins. But SmDFR also possesses flavanone 4-reductase (FNR) activity and can catalyze the conversion of eridictyol to luteoforol, EC 1.1.1.234 ?
-
?

Organism

Organism UniProt Comment Textmining
Saussurea medusa A5Z0G1 gene SmDFR
-
Saussurea medusa A5Z0G1 putative
-

Source Tissue

Source Tissue Comment Organism Textmining
callus a red callus line Saussurea medusa
-
flower
-
Saussurea medusa
-
flower expression in flowers is about 8fold higher compared with leaves and roots Saussurea medusa
-
leaf
-
Saussurea medusa
-
leaf expression in flowers is about 8fold higher compared with leaves and roots Saussurea medusa
-
additional information expression level of SmDFR is higher in flowers compared with both leaves and roots Saussurea medusa
-
root
-
Saussurea medusa
-
root expression in flowers is about 8fold higher compared with leaves and roots Saussurea medusa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-dihydrokaempferol + NADPH + H+
-
Saussurea medusa cis-3,4-leucopelargonidin + NADP+
-
?
(+)-dihydroquercetin + NADPH + H+
-
Saussurea medusa cis-3,4-leucocyanidin + NADP+
-
?
dihydroquercetin + NADPH + H+
-
Saussurea medusa leucocyanidin + NADP+
-
?
additional information the enzyme catalyzes the reduction of dihydroflavonols to leucoanthocyanins. But SmDFR also possesses flavanone 4-reductase (FNR) activity and can catalyze the conversion of eridictyol to luteoforol, EC 1.1.1.234 Saussurea medusa ?
-
?
additional information no activity in reduction of dihydromyricetin Saussurea medusa ?
-
?
additional information no substrate: dihydromyricetin. enzyme additionally shows flavanone-4-reductase activity, EC 1.1.1.234 Saussurea medusa ?
-
?

Subunits

Subunits Comment Organism
? x * 38600, SDS-PAGE Saussurea medusa

Synonyms

Synonyms Comment Organism
DFR
-
Saussurea medusa
dihydroflavonol 4-reductase
-
Saussurea medusa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Saussurea medusa

General Information

General Information Comment Organism
evolution the enzyme belongs to the plant DFR superfamily, phylogenetic analysis Saussurea medusa
metabolism the DFR gene is a key gene late in the flavonoid biosynthesis pathway, overview. The enzyme is a key enzyme in the biosynthesis of anthocyanidins, proanthocyanidins, and other flavonoids, and also possesses flavanone 4-reductase activity Saussurea medusa
physiological function the enzyme is of importance in plant development Saussurea medusa