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Literature summary for 1.1.1.218 extracted from
- Characterization of hamster NAD+-dependent 3(17)beta-hydroxysteroid dehydrogenase belonging to the aldo-keto reductase 1C subfamily (2015), J. Biochem., 158, 425-434.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene AKR1C34, cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Mesocricetus auratus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hexestrol | - |
Mesocricetus auratus |  |
| hinokitiol | - |
Mesocricetus auratus | |
| additional information | no or poor inhibition by 7-hydroxyflavone, chrysin, quercetin, genistein, galangin, lithocholic acid, and zearalenone | Mesocricetus auratus | |
| Phenolphthalein | - |
Mesocricetus auratus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| morphine + NAD+ | Mesocricetus auratus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mesocricetus auratus | A0A097ZMY7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and dialysis | Mesocricetus auratus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | in male hamsters | Mesocricetus auratus | - |
| colon | in male hamsters | Mesocricetus auratus | - |
| heart | in female hamsters | Mesocricetus auratus | - |
| kidney | in male hamsters | Mesocricetus auratus | - |
| liver | - |
Mesocricetus auratus | - |
| additional information | mRNA for AKR1C34 is expressed liver-specific in female hamsters and ubiquitously in male hamsters | Mesocricetus auratus | - |
| small intestine | in male hamsters | Mesocricetus auratus | - |
| testis | in male hamsters | Mesocricetus auratus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme AKR1C34 shows 3alpha/17beta/20alpha-hydroxysteroid dehydrogenase activities, EC 1.1.1.239, detailed overview. Ala54 plays a critical role in non-/recognition of the steroidal substrates. Reaction products identification by thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS) | Mesocricetus auratus | ? | - |
? | |
| morphine + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
| trans-naphthalene dihydrodiol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AKR1C34 | - |
Mesocricetus auratus |
| morphine-6-dehydrogenase | UniProt | Mesocricetus auratus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mesocricetus auratus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mesocricetus auratus |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00065 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | Phenolphthalein | |
| 0.0013 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | Hexestrol | |
| 0.0032 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | hinokitiol | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | enzyme M6DH is a member of the aldoketo reductase (AKR) 1C subfamily in the AKR superfamily | Mesocricetus auratus |
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