Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | reversible | Cavia porcellus |
General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes during purification and storage, removal inactivates, reversible | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CdCl2 | dithiol modifier, strong competitive inhibitor to cofactor | Cavia porcellus | |
additional information | no inhibition by NEM, PCMB, iodoacetic acid | Cavia porcellus | |
Phenylarsine oxide | dithiol modifier, competitive inhibitor to cofactor binding and noncompetitively toward morphine binding | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
nalorphine | cosubstrate NAD+ | Cavia porcellus | |
0.033 | - |
codeine | with NADP+ | Cavia porcellus | |
0.044 | - |
normorphine | cosubstrate NAD+ | Cavia porcellus | |
0.048 | - |
nalorphine | cosubstrate NADP+ | Cavia porcellus | |
0.079 | - |
codeine | with NAD+ | Cavia porcellus | |
0.12 | - |
morphine | cosubstrate NAD+ | Cavia porcellus | |
0.15 | - |
normorphine | cosubstrate NADP+ | Cavia porcellus | |
0.4 | - |
ethylmorphine | cosubstrate NAD+ | Cavia porcellus | |
0.49 | - |
morphine | cosubstrate NADP+ | Cavia porcellus | |
0.54 | - |
Dihydrocodeine | cosubstrate NAD+ | Cavia porcellus | |
1.54 | - |
ethylmorphine | cosubstrate NADP+ | Cavia porcellus | |
2.11 | - |
Dihydrocodeine | cosubstrate NADP+ | Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Cavia porcellus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
gel filtration | Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Cavia porcellus Hartley | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, various chromatographic techniques | Cavia porcellus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
morphine + NAD(P)+ = morphinone + NAD(P)H + H+ | dehydrogenation of the 6-hydroxy group of morphine derivatives having a 7,8-unsaturated bond | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.53 | - |
morphine, NAD+ | Cavia porcellus |
Storage Stability | Organism |
---|---|
2-4°C, 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol, pH 6., 90% of the activity retained with both NAD+ and NADP+ for a week | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
codeine + NAD(P)+ | oxidation at 80% the rate of morphine oxidation | Cavia porcellus | codeinone + NAD(P)H | - |
? | |
codeine + NAD(P)+ | oxidation at 80% the rate of morphine oxidation | Cavia porcellus Hartley | codeinone + NAD(P)H | - |
? | |
dihydrocodeine + NADP+ | - |
Cavia porcellus | dihydrocodeinone + NADPH | - |
? | |
dihydrocodeine + NADP+ | - |
Cavia porcellus Hartley | dihydrocodeinone + NADPH | - |
? | |
ethylmorphine + NAD(P)+ | oxidation at 51% the rate of morphine oxidation | Cavia porcellus | ? | - |
? | |
morphine + NADP+ | - |
Cavia porcellus | morphinone + NADPH + H+ | production of morphinone demonstrated in vitro experiments | ? | |
morphine + NADP+ | - |
Cavia porcellus Hartley | morphinone + NADPH + H+ | production of morphinone demonstrated in vitro experiments | ? | |
nalorphine + NAD(P)+ | best substrate, oxidation at 120% the rate of morphine oxidation | Cavia porcellus | (5alpha)-3-hydroxy-17-(prop-2-en-1-yl)-7,8-didehydro-4,5-epoxymorphinan-6-one + NAD(P)H + H+ | - |
? | |
nalorphine + NAD(P)+ | best substrate, oxidation at 120% the rate of morphine oxidation | Cavia porcellus Hartley | (5alpha)-3-hydroxy-17-(prop-2-en-1-yl)-7,8-didehydro-4,5-epoxymorphinan-6-one + NAD(P)H + H+ | - |
? | |
normorphine + NAD(P)+ | about 3 times better substrate than morphine | Cavia porcellus | ? | - |
? | |
normorphine + NAD(P)+ | about 3 times better substrate than morphine | Cavia porcellus Hartley | ? | - |
? | |
trans-1,4-decalindiol + NADP+ | about 30% of the activity observed with morphine | Cavia porcellus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
enzyme assay | Cavia porcellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.1 | - |
with NADP+ as cofactor, in tricine-NaOH buffer | Cavia porcellus |
9.7 | - |
with NAD+ as cofactor, in glycine-NaCl-NaOH buffer | Cavia porcellus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
in 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol + NADP+ or NAD+ at least for a week stable at 2-4°C | Cavia porcellus |
8 | - |
in 50 mM sodium phosphate buffer + 100 mM 2-mercaptoethanol + NAD+ at least for a week stable at 2-4°C | Cavia porcellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | utilized both with nearly equal efficiency | Cavia porcellus |