Application | Comment | Organism |
---|---|---|
agriculture | recombinant farnesol dehydrogenase may provide a useful molecular tool in manipulating juvenile hormone biosynthesis to generate transgenic plants for pest control | Persicaria minor |
biotechnology | recombinant farnesol dehydrogenase may provide a useful molecular tool in manipulating juvenile hormone biosynthesis to generate transgenic plants for pest control | Persicaria minor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | complete inhibition at 1 mM | Persicaria minor | |
2,2-dipyridyl | 52% inhibition at 1 mM | Persicaria minor | |
5,5'-dithiobis(2-nitrobenzoic acid) | complete inhibition at 1 mM | Persicaria minor | |
Ag+ | complete inhibition at 1 mM | Persicaria minor | |
Ca2+ | 24% inhibition at 1 mM | Persicaria minor | |
Cu2+ | complete inhibition at 1 mM | Persicaria minor | |
EDTA | complete inhibition at 1 mM | Persicaria minor | |
Fe3+ | complete inhibition at 1 mM | Persicaria minor | |
iodoacetamide | 66% inhibition at 1 mM | Persicaria minor | |
Li+ | complete inhibition at 1 mM | Persicaria minor | |
Mg2+ | 73% inhibition at 1 mM | Persicaria minor | |
additional information | the enzyme activity is inhibited by sulfhydryl reagents, metal-chelating agents and heavy metal ions | Persicaria minor | |
p-chloromercuribenzoate | complete inhibition at 1 mM | Persicaria minor | |
Sodium azide | 25% inhibition at 1 mM | Persicaria minor | |
Zn2+ | complete inhibition at 1 mM | Persicaria minor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Persicaria minor | |
0.17 | - |
(2E,6E)-farnesol | pH 9.5, 35°C | Persicaria minor | |
0.33 | - |
(2Z,6E)-farnesol | pH 9.5, 35°C | Persicaria minor | |
0.42 | - |
(2Z,6Z)-farnesol | pH 9.5, 35°C | Persicaria minor | |
0.71 | - |
carveol | pH 9.5, 35°C | Persicaria minor | |
0.74 | - |
NAD+ | pH 9.5, 35°C | Persicaria minor | |
0.77 | - |
(S)-perillyl alcohol | pH 9.5, 35°C | Persicaria minor | |
1 | - |
nerodilol | pH 9.5, 35°C | Persicaria minor | |
1.04 | - |
cinnamyl alcohol | pH 9.5, 35°C | Persicaria minor | |
12.5 | - |
geraniol | pH 9.5, 35°C | Persicaria minor | |
40 | - |
NADP+ | pH 9.5, 35°C | Persicaria minor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
- |
Persicaria minor |
70000 | - |
- |
Persicaria minor |
130000 | - |
gel filtration | Persicaria minor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Persicaria minor | - |
plants growing in an experimental plot at the Institute of Systems Biology of Universiti Kebangsaan Malaysia | - |
Purification (Comment) | Organism |
---|---|
native enzyme 234fold from leaves to apparent homogeneity by two different steps of anion-exchange chromatography and a cation exchange chromatography step, followed by three successive steps of gel filtration | Persicaria minor |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Persicaria minor | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.27 | - |
purified native enzyme, pH 9.5, 35°C | Persicaria minor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesol + NAD+ | preferred substrates | Persicaria minor | (2E,6E)-farnesal + NADH + H+ | - |
? | |
(2E,6E)-farnesol + NADP+ | NADP+ show 22% of the activity with NAD+ | Persicaria minor | (2E,6E)-farnesal + NADPH + H+ | - |
? | |
(2Z,6E)-farnesol + NAD+ | 66% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | (2Z,6E)-farnesal + NADH + H+ | - |
? | |
(2Z,6Z)-farnesol + NAD+ | 47% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | (2Z,6Z)-farnesal + NADH + H+ | - |
? | |
(S)-perillyl alcohol + NAD+ | 34% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | ? | - |
? | |
carveol + NAD+ | 32% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | ? | - |
? | |
cinnamyl alcohol + NAD+ | 35% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | ? | - |
? | |
geraniol + NAD+ | 37% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | ? | - |
? | |
additional information | trans,trans-farnesol is the preferred substrate for the Polygonum minus farnesol dehydrogenase, the geometrical isomers cis,trans-farnesol and cis,cis-farnesol are also oxidized by the enzyme with lower activity. No activity with nerol, p-cumic alcohol, borneol, linalool, menthol, beta-citronellol, 3,7-dihydrolinalool, benzyl alcohol, 2,4-octadien-1-ol, or 2,5-dimethyl-1.5-hexadien-3-ol, tetrahydrogeraniol, tetrahydrolinalool, tetrahydrolavandulol, ethanol, and methanol. Substrate specificity, overview | Persicaria minor | ? | - |
? | |
nerodilol + NAD+ | 36% of the activity with 2-trans,6-trans-farnesol | Persicaria minor | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 65000 + 1 * 70000, SDS-PAGE | Persicaria minor |
More | tryptic peptide mapping and MALDI-TOF/TOF-MS spectrometric analysis, sequence comparisons, overview | Persicaria minor |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 1.1.1.354 | Persicaria minor |
NAD(P)+-dependent farnesol dehydrogenase | - |
Persicaria minor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Persicaria minor |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | activity range, profile overview | Persicaria minor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | purified native enzyme, 10 min at pH 7.5 in 100 mM tricine-NaOH containing 2.5 mM 2-mercaptoethanol, completely stable up to 30°C, over 90% activity up to 55°C, loss of 80% activity at 60°C, loss of 85% activity at 65°C, and inactivation at 70°C | Persicaria minor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Persicaria minor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 11 | activity range, profile overview. At below pH 8.5 or above pH 10.0, the enzyme activity declines sharply | Persicaria minor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme utilizes both NAD+ and NADP+ with a strong preference for NAD+ | Persicaria minor | |
NAD+ | - |
Persicaria minor | |
NADP+ | - |
Persicaria minor |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Persicaria minor | isoelectric focusing | - |
6.8 |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of precursors that can be used for the biosynthetic pathway of juvenile hormone III (JH III, methyl-10R,11-epoxy-3,7,11-trimethyl-2E,6E-dodecadienoate). Deployment of transgenic plant with farnesol dehydrogenase enzyme will be beneficial for use in manipulating juvenile hormone biosynthesis in plants | Persicaria minor |