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Literature summary for 1.1.1.216 extracted from

  • Ahmad-Sohdi, N.A.; Seman-Kamarulzaman, A.F.; Mohamed-Hussein, Z.A.; Hassan, M.
    Purification and characterization of a novel NAD(P)+-farnesol dehydrogenase from Polygonum minus leaves (2015), PLoS ONE, 10, e0143310.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.216

Application

Application Comment Organism
agriculture recombinant farnesol dehydrogenase may provide a useful molecular tool in manipulating juvenile hormone biosynthesis to generate transgenic plants for pest control Persicaria minor
biotechnology recombinant farnesol dehydrogenase may provide a useful molecular tool in manipulating juvenile hormone biosynthesis to generate transgenic plants for pest control Persicaria minor

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition at 1 mM Persicaria minor
2,2-dipyridyl 52% inhibition at 1 mM Persicaria minor
5,5'-dithiobis(2-nitrobenzoic acid) complete inhibition at 1 mM Persicaria minor
Ag+ complete inhibition at 1 mM Persicaria minor
Ca2+ 24% inhibition at 1 mM Persicaria minor
Cu2+ complete inhibition at 1 mM Persicaria minor
EDTA complete inhibition at 1 mM Persicaria minor
Fe3+ complete inhibition at 1 mM Persicaria minor
iodoacetamide 66% inhibition at 1 mM Persicaria minor
Li+ complete inhibition at 1 mM Persicaria minor
Mg2+ 73% inhibition at 1 mM Persicaria minor
additional information the enzyme activity is inhibited by sulfhydryl reagents, metal-chelating agents and heavy metal ions Persicaria minor
p-chloromercuribenzoate complete inhibition at 1 mM Persicaria minor
Sodium azide 25% inhibition at 1 mM Persicaria minor
Zn2+ complete inhibition at 1 mM Persicaria minor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Persicaria minor
0.17
-
 (2E,6E)-farnesol pH 9.5, 35°C Persicaria minor
0.33
-
 (2Z,6E)-farnesol pH 9.5, 35°C Persicaria minor
0.42
-
 (2Z,6Z)-farnesol pH 9.5, 35°C Persicaria minor
0.71
-
 carveol pH 9.5, 35°C Persicaria minor
0.74
-
 NAD+ pH 9.5, 35°C Persicaria minor
0.77
-
 (S)-perillyl alcohol pH 9.5, 35°C Persicaria minor
1
-
 nerodilol pH 9.5, 35°C Persicaria minor
1.04
-
 cinnamyl alcohol pH 9.5, 35°C Persicaria minor
12.5
-
 geraniol pH 9.5, 35°C Persicaria minor
40
-
 NADP+ pH 9.5, 35°C Persicaria minor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
-
 Persicaria minor
70000
-
-
 Persicaria minor
130000
-
 gel filtration Persicaria minor

Organism

Organism UniProt Comment Textmining
Persicaria minor
-
 plants growing in an experimental plot at the Institute of Systems Biology of Universiti Kebangsaan Malaysia
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 234fold from leaves to apparent homogeneity by two different steps of anion-exchange chromatography and a cation exchange chromatography step, followed by three successive steps of gel filtration Persicaria minor

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Persicaria minor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.27
-
 purified native enzyme, pH 9.5, 35°C Persicaria minor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesol + NAD+ preferred substrates Persicaria minor (2E,6E)-farnesal + NADH + H+
-
 ?
(2E,6E)-farnesol + NADP+ NADP+ show 22% of the activity with NAD+ Persicaria minor (2E,6E)-farnesal + NADPH + H+
-
 ?
(2Z,6E)-farnesol + NAD+ 66% of the activity with 2-trans,6-trans-farnesol Persicaria minor (2Z,6E)-farnesal + NADH + H+
-
 ?
(2Z,6Z)-farnesol + NAD+ 47% of the activity with 2-trans,6-trans-farnesol Persicaria minor (2Z,6Z)-farnesal + NADH + H+
-
 ?
(S)-perillyl alcohol + NAD+ 34% of the activity with 2-trans,6-trans-farnesol Persicaria minor ?
-
 ?
carveol + NAD+ 32% of the activity with 2-trans,6-trans-farnesol Persicaria minor ?
-
 ?
cinnamyl alcohol + NAD+ 35% of the activity with 2-trans,6-trans-farnesol Persicaria minor ?
-
 ?
geraniol + NAD+ 37% of the activity with 2-trans,6-trans-farnesol Persicaria minor ?
-
 ?
additional information trans,trans-farnesol is the preferred substrate for the Polygonum minus farnesol dehydrogenase, the geometrical isomers cis,trans-farnesol and cis,cis-farnesol are also oxidized by the enzyme with lower activity. No activity with nerol, p-cumic alcohol, borneol, linalool, menthol, beta-citronellol, 3,7-dihydrolinalool, benzyl alcohol, 2,4-octadien-1-ol, or 2,5-dimethyl-1.5-hexadien-3-ol, tetrahydrogeraniol, tetrahydrolinalool, tetrahydrolavandulol, ethanol, and methanol. Substrate specificity, overview Persicaria minor ?
-
 ?
nerodilol + NAD+ 36% of the activity with 2-trans,6-trans-farnesol Persicaria minor ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 65000 + 1 * 70000, SDS-PAGE Persicaria minor
More tryptic peptide mapping and MALDI-TOF/TOF-MS spectrometric analysis, sequence comparisons, overview Persicaria minor

Synonyms

Synonyms Comment Organism
More cf. EC 1.1.1.354 Persicaria minor
NAD(P)+-dependent farnesol dehydrogenase
-
 Persicaria minor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Persicaria minor

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 activity range, profile overview Persicaria minor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 65 purified native enzyme, 10 min at pH 7.5 in 100 mM tricine-NaOH containing 2.5 mM 2-mercaptoethanol, completely stable up to 30°C, over 90% activity up to 55°C, loss of 80% activity at 60°C, loss of 85% activity at 65°C, and inactivation at 70°C Persicaria minor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Persicaria minor

pH Range

pH Minimum pH Maximum Comment Organism
7.5 11 activity range, profile overview. At below pH 8.5 or above pH 10.0, the enzyme activity declines sharply Persicaria minor

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme utilizes both NAD+ and NADP+ with a strong preference for NAD+ Persicaria minor
NAD+
-
 Persicaria minor
NADP+
-
 Persicaria minor

pI Value

Organism Comment pI Value Maximum pI Value
Persicaria minor isoelectric focusing
-
 6.8

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of precursors that can be used for the biosynthetic pathway of juvenile hormone III (JH III, methyl-10R,11-epoxy-3,7,11-trimethyl-2E,6E-dodecadienoate). Deployment of transgenic plant with farnesol dehydrogenase enzyme will be beneficial for use in manipulating juvenile hormone biosynthesis in plants Persicaria minor