Application | Comment | Organism |
---|---|---|
synthesis | ga2dh-overexpressing strain Gluconobacter oxydans_tufB_ga2dh is a useful species for use in 2-dehydro-D-gluconate production | Gluconobacter oxydans |
Cloned (Comment) | Organism |
---|---|
gene ga2dh, recombinant overexpression of the enzyme in Gluconobacter oxydans strain DSM2003 under the control of three promoters, PtufB, Pga2dh or Pghp0169, respectively. Recombinant strain Gluconobacter oxydans_tufB_ga2dh shows a similar growth rate to that of the control strain and displays the highest specific productivity of 2-dehydro-D-gluconate from D-gluconate, which is increased nearly twofold compared with that of the control strain during batch biotransformation, method optimization | Gluconobacter oxydans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | development of an efficient bacterial strain of Gluconobacter oxydans_tufB_ga2dh for the production of 2-dehydro-D-gluconate by overexpressing the ga2dh gene in Gluconobacter oxydans. Supply of sufficient oxygen enhances the positive effect of gene overexpression on 2-dehydro-D-gluconate production. Gluconobacter oxydans_tufB_ga2dh is a competitive species for use in 2-dehydro-D-gluconate production. Transgenic strains, evaluation and optimization, detailed overview | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Gluconobacter oxydans | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate + NADP+ | Gluconobacter oxydans | - |
2-dehydro-D-gluconate + NADPH + H+ | - |
? | |
D-gluconate + NADP+ | Gluconobacter oxydans 621H | - |
2-dehydro-D-gluconate + NADPH + H+ | - |
? | |
additional information | Gluconobacter oxydans | for large-scale production of 2-dehydro-D-gluconate, the cells require for formation of 2-dehydro-D-gluconate from D-gluconate oxygen as the final acceptor of electrons formed during the oxidation ofD -gluconate | ? | - |
? | |
additional information | Gluconobacter oxydans 621H | for large-scale production of 2-dehydro-D-gluconate, the cells require for formation of 2-dehydro-D-gluconate from D-gluconate oxygen as the final acceptor of electrons formed during the oxidation ofD -gluconate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | Q5FRK5 | - |
- |
Gluconobacter oxydans 621H | Q5FRK5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gluconate + NADP+ | - |
Gluconobacter oxydans | 2-dehydro-D-gluconate + NADPH + H+ | - |
? | |
D-gluconate + NADP+ | - |
Gluconobacter oxydans 621H | 2-dehydro-D-gluconate + NADPH + H+ | - |
? | |
additional information | for large-scale production of 2-dehydro-D-gluconate, the cells require for formation of 2-dehydro-D-gluconate from D-gluconate oxygen as the final acceptor of electrons formed during the oxidation ofD -gluconate | Gluconobacter oxydans | ? | - |
? | |
additional information | for large-scale production of 2-dehydro-D-gluconate, the cells require for formation of 2-dehydro-D-gluconate from D-gluconate oxygen as the final acceptor of electrons formed during the oxidation ofD -gluconate | Gluconobacter oxydans 621H | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GA2DH | - |
Gluconobacter oxydans |
gluconate-2-dehydrogenase | - |
Gluconobacter oxydans |
GOX1230 | - |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Gluconobacter oxydans |