Crystallization (Comment) | Organism |
---|---|
- |
Acetobacter pasteurianus |
- |
Gluconacetobacter liquefaciens |
- |
Acetobacter ascendens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | complete inhibition | Acetobacter ascendens | |
Hg2+ | complete inhibition | Acetobacter pasteurianus | |
Hg2+ | complete inhibition | Gluconacetobacter liquefaciens | |
Hg2+ | complete inhibition | Gluconobacter oxydans | |
sulfhydryl reagents | strong | Acetobacter ascendens | |
sulfhydryl reagents | strong | Acetobacter pasteurianus | |
sulfhydryl reagents | strong | Gluconacetobacter liquefaciens | |
sulfhydryl reagents | strong | Gluconobacter oxydans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Gluconobacter oxydans | 5737 | - |
cytoplasm | - |
Acetobacter pasteurianus | 5737 | - |
cytoplasm | - |
Gluconacetobacter liquefaciens | 5737 | - |
cytoplasm | - |
Acetobacter ascendens | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal requirement | Gluconobacter oxydans | |
additional information | no metal requirement | Acetobacter pasteurianus | |
additional information | no metal requirement | Gluconacetobacter liquefaciens | |
additional information | no metal requirement | Acetobacter ascendens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 43000 + 1 * 34000, SDS-PAGE | Gluconacetobacter liquefaciens |
43000 | - |
2 * 43000 + 1 * 34000, SDS-PAGE | Gluconacetobacter liquefaciens |
120000 | - |
gel filtration | Acetobacter pasteurianus |
120000 | - |
gel filtration | Gluconacetobacter liquefaciens |
120000 | - |
gel filtration | Acetobacter ascendens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-D-gluconate + NADPH | Acetobacter pasteurianus | - |
D-gluconate + NADP+ | - |
r | |
2-dehydro-D-gluconate + NADPH | Gluconobacter oxydans | reverse reaction at the same rate by Gluconobacter enzyme | D-gluconate + NADP+ | - |
r | |
2-dehydro-D-gluconate + NADPH | Gluconacetobacter liquefaciens | reverse reaction at the same rate by Gluconobacter enzyme | D-gluconate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter ascendens | - |
- |
- |
Acetobacter pasteurianus | - |
- |
- |
Gluconacetobacter liquefaciens | - |
- |
- |
Gluconobacter oxydans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gluconacetobacter liquefaciens |
affinity chromatography on Blue-Dextran Sepharose B | Acetobacter ascendens |
partial | Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
61.03 | - |
- |
Gluconacetobacter liquefaciens |
187 | - |
- |
Acetobacter ascendens |
336 | - |
- |
Acetobacter pasteurianus |
Storage Stability | Organism |
---|---|
5°C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol | Gluconobacter oxydans |
5°C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol | Acetobacter pasteurianus |
5°C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol | Gluconacetobacter liquefaciens |
5°C, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol | Acetobacter ascendens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-D-gluconate + NADPH | - |
Acetobacter pasteurianus | D-gluconate + NADP+ | - |
r | |
2-dehydro-D-gluconate + NADPH | i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter | Gluconobacter oxydans | D-gluconate + NADP+ | - |
r | |
2-dehydro-D-gluconate + NADPH | i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter | Gluconacetobacter liquefaciens | D-gluconate + NADP+ | - |
r | |
2-dehydro-D-gluconate + NADPH | i.e. 2-keto-D-gluconate, best substrate for Acetobacter ascendens and Gluconobacter | Acetobacter ascendens | D-gluconate + NADP+ | - |
r | |
2-dehydro-D-gluconate + NADPH | strongly preferred reaction of Acetobacter enzyme | Acetobacter pasteurianus | D-gluconate + NADP+ | - |
r | |
2-dehydro-D-gluconate + NADPH | strongly preferred reaction of Acetobacter enzyme | Acetobacter ascendens | D-gluconate + NADP+ | - |
r | |
2-dehydro-D-gluconate + NADPH | reverse reaction at the same rate by Gluconobacter enzyme | Gluconobacter oxydans | D-gluconate + NADP+ | - |
r | |
2-dehydro-D-gluconate + NADPH | reverse reaction at the same rate by Gluconobacter enzyme | Gluconacetobacter liquefaciens | D-gluconate + NADP+ | - |
r | |
2-keto-L-gulonate + NADPH | third best substrate | Gluconobacter oxydans | L-idonate + NADP+ | - |
r | |
2-keto-L-gulonate + NADPH | third best substrate | Gluconacetobacter liquefaciens | L-idonate + NADP+ | - |
r | |
2-keto-L-gulonate + NADPH | third best substrate | Acetobacter ascendens | L-idonate + NADP+ | - |
r | |
2-keto-L-gulonate + NADPH | betst substrate | Acetobacter pasteurianus | L-idonate + NADP+ | - |
r | |
5-keto-D-gluconate + NADPH | - |
Gluconobacter oxydans | D-gluconate + NADP+ | - |
? | |
5-keto-D-gluconate + NADPH | - |
Gluconacetobacter liquefaciens | D-gluconate + NADP+ | - |
? | |
5-keto-D-gluconate + NADPH | poor substrate | Acetobacter pasteurianus | D-gluconate + NADP+ | - |
? | |
5-keto-D-gluconate + NADPH | poor substrate | Acetobacter ascendens | D-gluconate + NADP+ | - |
? | |
D-galactonate + NADP+ | - |
Acetobacter pasteurianus | 2-dehydro-D-galactonate + NADPH | - |
r | |
D-galactonate + NADP+ | - |
Acetobacter ascendens | 2-dehydro-D-galactonate + NADPH | - |
r | |
D-galactonate + NADP+ | third best substrate | Gluconobacter oxydans | 2-dehydro-D-galactonate + NADPH | - |
r | |
D-galactonate + NADP+ | third best substrate | Gluconacetobacter liquefaciens | 2-dehydro-D-galactonate + NADPH | - |
r | |
D-xylonate + NADPH | - |
Gluconobacter oxydans | 2-keto-D-xylonate + NADP+ | - |
? | |
D-xylonate + NADPH | - |
Acetobacter pasteurianus | 2-keto-D-xylonate + NADP+ | - |
? | |
D-xylonate + NADPH | - |
Gluconacetobacter liquefaciens | 2-keto-D-xylonate + NADP+ | - |
? | |
L-idonate + NADP+ | poor substrate | Acetobacter pasteurianus | 2-keto-L-idonate + NADPH | - |
? | |
L-idonate + NADP+ | poor substrate | Acetobacter ascendens | 2-keto-L-idonate + NADPH | - |
? | |
L-idonate + NADP+ | best substrate | Gluconobacter oxydans | 2-keto-L-idonate + NADPH | - |
? | |
L-idonate + NADP+ | best substrate | Gluconacetobacter liquefaciens | 2-keto-L-idonate + NADPH | - |
? | |
additional information | no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate | Gluconobacter oxydans | ? | - |
? | |
additional information | no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate | Acetobacter pasteurianus | ? | - |
? | |
additional information | no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate | Gluconacetobacter liquefaciens | ? | - |
? | |
additional information | no substrates are: 6-phospho-D-gluconate, D-mannonate, D-arabonate | Acetobacter ascendens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | - |
Acetobacter ascendens |
trimer | 2 * 43000 + 1 * 34000, SDS-PAGE | Gluconacetobacter liquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
stable for 5 min, enhanced by the addition of substrate | Gluconobacter oxydans |
55 | - |
stable for 5 min, enhanced by the addition of substrate | Acetobacter pasteurianus |
55 | - |
stable for 5 min, enhanced by the addition of substrate | Gluconacetobacter liquefaciens |
55 | - |
stable for 5 min, enhanced by the addition of substrate | Acetobacter ascendens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of 2-ketogluconate | Gluconobacter oxydans |
6 | - |
reduction of 2-ketogluconate | Acetobacter pasteurianus |
6 | - |
reduction of 2-ketogluconate | Gluconacetobacter liquefaciens |
6 | - |
reduction of 2-ketogluconate | Acetobacter ascendens |
10.5 | - |
- |
Acetobacter ascendens |
10.5 | - |
oxidation of gluconate | Gluconacetobacter liquefaciens |
11 | - |
oxidation of gluconate | Acetobacter ascendens |
12 | - |
oxidation of gluconate | Acetobacter pasteurianus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5°C | Gluconobacter oxydans |
6 | - |
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5°C | Acetobacter pasteurianus |
6 | - |
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5°C | Gluconacetobacter liquefaciens |
6 | - |
crude and crystallized, stable in 0.01 M potassium phosphate buffer with the addition of 2-mercaptoethanol at 5°C | Acetobacter ascendens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADH | Gluconobacter oxydans | |
additional information | no activity with NADH | Acetobacter pasteurianus | |
additional information | no activity with NADH | Gluconacetobacter liquefaciens | |
additional information | no activity with NADH | Acetobacter ascendens | |
NADP+ | - |
Gluconobacter oxydans | |
NADP+ | - |
Acetobacter pasteurianus | |
NADP+ | - |
Gluconacetobacter liquefaciens | |
NADP+ | - |
Acetobacter ascendens | |
NADPH | - |
Gluconobacter oxydans | |
NADPH | - |
Acetobacter pasteurianus | |
NADPH | - |
Gluconacetobacter liquefaciens | |
NADPH | - |
Acetobacter ascendens |